Migliaccio, Nunzia
(2015)
BIOSILICA NANOVECTOR FROM DIATOMITE FOR siRNA
TRANSPORT IN CANCER CELL.
[Tesi di dottorato]
| Item Type: |
Tesi di dottorato
|
| Lingua: |
English |
| Title: |
BIOSILICA NANOVECTOR FROM DIATOMITE FOR siRNA
TRANSPORT IN CANCER CELL |
| Creators: |
| Creators | Email |
|---|
| Migliaccio, Nunzia | nunzia.migliaccio@unina.it |
|
| Date: |
27 March 2015 |
| Number of Pages: |
46 |
| Institution: |
Università degli Studi di Napoli Federico II |
| Department: |
Medicina Molecolare e Biotecnologie Mediche |
| Scuola di dottorato: |
Medicina molecolare |
| Dottorato: |
Biochimica e biologia cellulare e molecolare |
| Ciclo di dottorato: |
26 |
| Coordinatore del Corso di dottorato: |
| nome | email |
|---|
| Arcari, Paolo | arcari@unina.it |
|
| Tutor: |
| nome | email |
|---|
| Lamberti, Annalisa | UNSPECIFIED |
|
| Date: |
27 March 2015 |
| Number of Pages: |
46 |
| Uncontrolled Keywords: |
Diatomite; Drug delivery; siRNA |
| Settori scientifico-disciplinari del MIUR: |
Area 05 - Scienze biologiche > BIO/10 - Biochimica |
| Aree tematiche (7° programma Quadro): |
NANOSCIENZE, NANOTECNOLOGIE, MATERIALE E PRODUZIONE > Nanoscienze e Nanotecnologie
NANOSCIENZE, NANOTECNOLOGIE, MATERIALE E PRODUZIONE > Materiali |
[error in script]
[error in script]
| Date Deposited: |
09 Apr 2015 05:53 |
| Last Modified: |
24 Sep 2015 10:04 |
| URI: |
http://www.fedoa.unina.it/id/eprint/10145 |
| DOI: |
10.6092/UNINA/FEDOA/10145 |
Abstract
Diatomite is a natural porous biomaterial of sedimentary origin,
formed by fragments of diatom siliceous skeletons, called "frustules".
Due to large availability in many areas of the world, chemical
stability, and non-toxicity, these fossil structures have been
widespread used in a lot of industrial applications, such as food
production, water extracting agent, production of cosmetics and
pharmaceutics. However, diatomite is surprisingly still rarely used in
biomedical applications.
In this study, the properties of diatomite nanoparticles as potential
system for the delivery of anticancer molecules in cancer cells were
exploited.
A purification procedure, based on thermal treatments in strong acid
solutions, was used to remove inorganic and organic impurities from
diatomite and to make them a safe material for medical applications.
The micrometric diatomite powder was reduced in nanoparticles by
mechanical crushing, sonication, and filtering.
Morphology and composition of diatomite were investigated by scanning electron microscopy equipped by energy dispersive X-ray spectroscopy, photoluminescence and dynamic light scattering measurements. In-vitro experiments show a very low toxicity on exposure of the cells to diatomite nanoparticle concentration up to 300 μg/ml for 72 h.
Diatomite nanoparticles were functionalized by 3-aminopropyltriethoxysilane and labeled by tetramethylrhodamine
isothiocyanate. Different concentrations of chemically modified
nanoparticles were incubated with cancer cells and confocal microscopy was performed. Imaging analysis showed an efficient cellular uptake and homogeneous distribution of nanoparticles in cytoplasm and nucleus, thus suggesting their potentiality as nanocarriers for drug delivery.
Subsequently, siRNA bioconjugation were performed and confocal
microscopy imaging on cancer cells incubated with siRNAconjugated
nanoparticles demonstrated a cytoplasmatic localization of vectors. Gene silencing by delivered siRNA was also demonstrated.
Our studies endorse diatomite nanoparticles as non-toxic nanocarriers
for siRNA transport in cancer cells. siRNA-diatomite nanoconjugate
may be well suited for delivery of therapeutic to cancer cells.
Downloads per month over past year
Actions (login required)
 |
View Item |
