Meccariello, Angela
(2017)
Genomics, transcriptomics and CRISPR/CAS9 gene
editing approaches to investigate embryonic sex
determination in insect pest Ceratitis capitata.
[Tesi di dottorato]
| Item Type: |
Tesi di dottorato
|
| Lingua: |
English |
| Title: |
Genomics, transcriptomics and CRISPR/CAS9 gene
editing approaches to investigate embryonic sex
determination in insect pest Ceratitis capitata. |
| Creators: |
| Creators | Email |
|---|
| Meccariello, Angela | angela.meccariello@unina.it |
|
| Date: |
8 April 2017 |
| Number of Pages: |
101 |
| Institution: |
Università degli Studi di Napoli Federico II |
| Department: |
Biologia |
| Dottorato: |
Biologia |
| Ciclo di dottorato: |
29 |
| Coordinatore del Corso di dottorato: |
| nome | email |
|---|
| Cozzolino, Salvatore | salvatore.cozzolino@unina.it |
|
| Tutor: |
| nome | email |
|---|
| Saccone, Giuseppe | UNSPECIFIED |
|
| Date: |
8 April 2017 |
| Number of Pages: |
101 |
| Uncontrolled Keywords: |
Transcriptomic, insects, sex determination |
| Settori scientifico-disciplinari del MIUR: |
Area 05 - Scienze biologiche > BIO/18 - Genetica |
[error in script]
[error in script]
| Date Deposited: |
20 Apr 2017 11:37 |
| Last Modified: |
16 Mar 2018 13:15 |
| URI: |
http://www.fedoa.unina.it/id/eprint/11659 |
| DOI: |
10.6093/UNINA/FEDOA/11659 |
Abstract
During my PhD, I have carried out studies of genomic and transcriptomic in the non-model system Ceratitis
capitata. C. capitata (Diptera, Tephritidae), also called “medfly”, is an insect pest that spread over almost
every continent and represents a great danger to the cultivation of fruits for human alimentation. Its vast
diffusion, together with its ability to parasitize many fruit hosts and the difficulties humans encountered
when trying to control and eradicate it from local areas, made C. capitata one of the most dangerous species
to agriculture on a global scale, and therefore, to a significant portion of national and international
economies. The reproduction and the sex determination are two crucial aspects to understand the biology of
insects and represent optimal targets to limit their growth and diffusion.
The project of my PhD consists into two parallel and synergic research lines:
1) transcriptomic (Illumina®) and genomic (PacBio®) approaches to the identification of novel Y-linked
genes potentially involved in male sex determination, and X-linked genes in “medfly”;
2) the development and perfecting of a powerful technique of gene editing, the CRISPR/Cas9, to study
these novel gene functions.
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