Schiattarella, Gabriele Giacomo (2017) Unfolded Protein Response Signaling Pathway Regulates Cardiac Function in Heart Failure with Preserved Ejection Fraction. [Tesi di dottorato]
Preview |
Text
Schiattarella_Gabriele_Tesi.pdf Download (4MB) | Preview |
| Item Type: | Tesi di dottorato |
|---|---|
| Resource language: | English |
| Title: | Unfolded Protein Response Signaling Pathway Regulates Cardiac Function in Heart Failure with Preserved Ejection Fraction |
| Creators: | Creators Email Schiattarella, Gabriele Giacomo schiattarella@unina.it |
| Date: | 27 November 2017 |
| Number of Pages: | 40 |
| Institution: | Università degli Studi di Napoli Federico II |
| Department: | dep18 |
| Dottorato: | phd053 |
| Ciclo di dottorato: | 30 |
| Coordinatore del Corso di dottorato: | nome email Marone, Gianni marone@unina.it |
| Tutor: | nome email Esposito, Giovanni UNSPECIFIED |
| Date: | 27 November 2017 |
| Number of Pages: | 40 |
| Keywords: | Heart failure, Diastolic function, Animal models, Unfolded protein response, iNOS, XBP1s |
| Settori scientifico-disciplinari del MIUR: | Area 06 - Scienze mediche > MED/11 - Malattie dell'apparato cardiovascolare |
| Date Deposited: | 21 Dec 2017 09:03 |
| Last Modified: | 12 Apr 2019 09:12 |
| URI: | http://www.fedoa.unina.it/id/eprint/12013 |
Collection description
Heart failure with preserved ejection fraction (HFpEF) is a clinical syndrome effecting more than 3 million people in the United States alone. HFpEF patients have a 5-year survival rate of less than 50% with no change in prognosis over the last 30 years. The lack of clinical treatments point to an urgent need for mechanistic studies of HFpEF pathogenesis. The cardiac remodeling and dysfunction in HFpEF is driven by comorbidities such as obesity, diabetes, arterial hypertension and endothelial dysfunction which lead to systemic inflammation. Here we report that, in mice, simultaneous metabolic and hypertensive stress, through the combination of high fat diet (HFD) and constitutive nitric oxide (NO) synthase inhibition by administration of N[w]-nitro-l-arginine methyl ester (L-NAME) recapitulates systemic and cardiovascular alterations of human HFpEF. The heart failure phenotype was only observed under combined treatment with HFD and L-NAME and was associated with molecular changes within the cardiomyocytes. Specifically, the spliced form of X-box binding protein 1 (Xbp1s), a downstream effector of the unfolded protein response (UPR), exhibited a significant reduction in the HFpEF model hearts and cardiomyocytes. This decrease in Xbp1s was also observed in human HFpEF hearts. Reduction in Xbp1s was caused by increased inducible NO synthase (iNOS) activity and S-nitrosylation of IRE1a, which ultimately led to a defect in XBP1 splicing activity. Importantly, cardiomyocyte-specific inducible overexpression of Xbp1s partially ameliorated diastolic dysfunction, exercise intolerance and pulmonary congestion in HFpEF mice. We have developed a novel preclinical model of HFpEF, unveiling iNOS-driven dysregulation of Xbp1s as a crucial mechanism of cardiomyocyte dysfunction. These results demonstrate the essential role of protein homeostasis dysregulation in HFpEF cardiomyocytes and suggest that inhibiting iNOS activity may be a viable therapeutic strategy in this condition.
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |
