Di Gennaro, Mariagrazia (2017) Construction of self assembling scaffold of decellularized cardiac ecm and fibrin for the treatment of myocardial infarction. [Tesi di dottorato]


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Item Type: Tesi di dottorato
Resource language: English
Title: Construction of self assembling scaffold of decellularized cardiac ecm and fibrin for the treatment of myocardial infarction
Di Gennaro, Mariagraziamariagraziadig@libero.it
Date: 29 November 2017
Number of Pages: 96
Institution: Università degli Studi di Napoli Federico II
Department: dep17
Dottorato: phd069
Ciclo di dottorato: 30
Coordinatore del Corso di dottorato:
Montagnani, Stefaniamontagna@unina.it
Di Meglio, FrancaUNSPECIFIED
Date: 29 November 2017
Number of Pages: 96
Keywords: stem cells; fibrin gels; cardiac regeneration
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/16 - Anatomia umana
Date Deposited: 19 Dec 2017 06:52
Last Modified: 12 Apr 2019 09:06
URI: http://www.fedoa.unina.it/id/eprint/12023

Collection description

Cardiac tissue engineering aims at restoring cell compartment along with myocardial extracellular microenvironment. Even though countless combinations of synthetic or biological scaffolds and stem/progenitor cells of various origin have been tested so far, the most suitable candidate is yet to be found. With the aim to develop a natural injectable self-assembling scaffold able to be serve as both three-dimensional platform and stem/progenitor cell delivery method, we assembled fibrin gels incorporating Cardiac Primitive Cells (CPC) and cardiac decellularized extracellular matrix (d-ECM). Cryosections of cardiac ECM were decellularized and d-ECM was lyophilized and solubilized. d-ECM solution was mixed with the fibrin solution carrying Cardiac Primitive Cells (CPC) isolated from adult heart and allowed to gel at 37°C. Several Fibrin to Matrix ratios (F: M) were tested to determine the ideal composition in terms of time of gelling and three-dimensional (3D) architecture. Gels were cultured for three days, then fixed and processed as tissues for histological study. Histochemistry revealed the presence of viable CPC in the gels whose architecture varied from densely packed to very loose. Due to time of gelling and to concentration of fibrin, the distribution of CPC in the scaffold was even only in the gel with F:M of 1:1. According to our results, the combination of CPC with fibrin and d-ECM at F:M of 1:1, being injectable and self-assembling at body temperature, provides an attracting alternate to bioconstructs.


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