Cimmino, Chiara (2021) Engineering patterned and dynamic surfaces for the spatio-temporal control of cell behaviour. [Tesi di dottorato]


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Item Type: Tesi di dottorato
Resource language: English
Title: Engineering patterned and dynamic surfaces for the spatio-temporal control of cell behaviour
Date: 15 July 2021
Number of Pages: 168
Institution: Università degli Studi di Napoli Federico II
Department: Ingegneria Chimica, dei Materiali e della Produzione Industriale
Dottorato: Ingegneria dei prodotti e dei processi industriali
Ciclo di dottorato: 33
Coordinatore del Corso di dottorato:
Ventre, MaurizioUNSPECIFIED
Netti, Paolo AntonioUNSPECIFIED
Date: 15 July 2021
Number of Pages: 168
Keywords: Azobenzene, cell shape, chromatin conformation
Settori scientifico-disciplinari del MIUR: Area 09 - Ingegneria industriale e dell'informazione > ING-IND/34 - Bioingegneria industriale
Date Deposited: 20 Jul 2021 12:04
Last Modified: 07 Jun 2023 11:25

Collection description

Stem cell shape and mechanical properties in vitro can be directed by geometrically defined micropatterned adhesion substrates. However conventional methods are limited by the fixed micropattern design, which cannot recapitulate the dynamic changes of the natural cell microenvironment. Recent advancements in microfabrication technologies in combination with the use of light-responsive materials, allow to manipulate the shape of living cells in real-time in a non-invasive Spatio-temporal controlled way to introduce additional geometrically defined adhesion sites and to study relative cell behaviour. Here, the confocal laser technique is exploited for dynamically evaluate the variation over time of the tensional and morphological cell state. This method allows the precise control of specific actin structures that regulate cell architecture. Actin filament bundles, initially randomly organized in circular-shaped cells, are induced to align and distribute to form a rectangular-shaped cell in response to specific dynamic changes in the cell adhesion pattern. The changes in morphology also reflect dramatic changes in FAs distribution, cell mechanics, nuclear morphology, and chromatin conformation. The reported strategy is convenient to explore the cell-substrate interface and the mechanisms through which cell geometry regulates cell signalling in a facile and cost-effective manner and it open new routes to understand how the field of dynamic platforms should potentially contribute to unveil complex biological events such as the modulation of cell shape.


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