Bruno, Cristina (2022) Functional foods for infectious diseases: protective action elicited by a postbiotic derived from cow milk fermentation with Lacticaseibacillus paracasei CBAL74 against Rotavirus and SARS-CoV-2. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Resource language: English
Title: Functional foods for infectious diseases: protective action elicited by a postbiotic derived from cow milk fermentation with Lacticaseibacillus paracasei CBAL74 against Rotavirus and SARS-CoV-2
Creators:
CreatorsEmail
Bruno, Cristinacristina.bruno@unina.it
Date: 14 March 2022
Number of Pages: 96
Institution: Università degli Studi di Napoli Federico II
Department: Agraria
Dottorato: Scienze agrarie e agroalimentari
Ciclo di dottorato: 34
Coordinatore del Corso di dottorato:
nomeemail
BARONE, AMALIAambarone@unina.it
Tutor:
nomeemail
BERNI CANANI, ROBERTOUNSPECIFIED
Date: 14 March 2022
Number of Pages: 96
Keywords: viral infections, gut barrier, functional foods
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 05 - Scienze biologiche > BIO/19 - Microbiologia generale
Area 06 - Scienze mediche > MED/12 - Gastroenterologia
Date Deposited: 18 Mar 2022 10:42
Last Modified: 28 Feb 2024 11:00
URI: http://www.fedoa.unina.it/id/eprint/14407

Collection description

Emerging evidence suggest the efficacy of the postbiotic derived from cow's milk fermentation with the probiotic Lacticaseibacillus paracasei CBA L74 (FM-CBAL74) in preventing pediatric infectious diseases. Rotavirus (RV) is a non-enveloped double-stranded RNA (dsRNA) virus that is the leading cause of acute gastroenteritis-associated morbidity and mortality in early childhood. RV is responsible >500.000 deaths and approximately 2.4 million hospitalization in young children worldwide annually. SARS-CoV-2 is a single-stranded RNA (ssRNA) virus responsible for the Coronavirus disease (COVID-19) pandemic responsible for around 5.6 million deaths and more than 110 million cases reported globally in the last 2 years. Here, we investigated the protective action elicited by FM-CBAL74 against RV and SARS-CoV-2 infection in an in vitro model of human gut epithelium. We demonstrated that pre-incubation with FM-CBAL74 inhibited RV-induced epithelial barrier damage (tight junctions (TJs) and adherent junctions’ protein (AJ), and transepithelial electrical resistance evaluation), reactive oxygen species (ROS) and pro-inflammatory cytokines (IL-6, IL-8, TNF-α) release, through MAP kinases pathway activation. Regarding SARS-CoV-2 infection, we demonstrated that pre- incubation with FM-CBA L74 reduced the number of infected cells, the expression of the host functional receptor angiotensin-converting enzyme-2 (ACE2) and the pro-inflammatory cytokines IL-6, VEGFB, IL-15, IL-1β release. Modulating immune and non-immune protective mechanisms, the postbiotic FM-CBAL74 exerts a protective action against RV and SARS-CoV-2 infection. These findings may open new preventive and therapeutic strategies for these conditions.

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