Bruno, Cristina (2022) Functional foods for infectious diseases: protective action elicited by a postbiotic derived from cow milk fermentation with Lacticaseibacillus paracasei CBAL74 against Rotavirus and SARS-CoV-2. [Tesi di dottorato]
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| Item Type: | Tesi di dottorato |
|---|---|
| Resource language: | English |
| Title: | Functional foods for infectious diseases: protective action elicited by a postbiotic derived from cow milk fermentation with Lacticaseibacillus paracasei CBAL74 against Rotavirus and SARS-CoV-2 |
| Creators: | Creators Email Bruno, Cristina cristina.bruno@unina.it |
| Date: | 14 March 2022 |
| Number of Pages: | 96 |
| Institution: | Università degli Studi di Napoli Federico II |
| Department: | Agraria |
| Dottorato: | Scienze agrarie e agroalimentari |
| Ciclo di dottorato: | 34 |
| Coordinatore del Corso di dottorato: | nome email BARONE, AMALIA ambarone@unina.it |
| Tutor: | nome email BERNI CANANI, ROBERTO UNSPECIFIED |
| Date: | 14 March 2022 |
| Number of Pages: | 96 |
| Keywords: | viral infections, gut barrier, functional foods |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 05 - Scienze biologiche > BIO/19 - Microbiologia generale Area 06 - Scienze mediche > MED/12 - Gastroenterologia |
| Date Deposited: | 18 Mar 2022 10:42 |
| Last Modified: | 28 Feb 2024 11:00 |
| URI: | http://www.fedoa.unina.it/id/eprint/14407 |
Collection description
Emerging evidence suggest the efficacy of the postbiotic derived from cow's milk fermentation with the probiotic Lacticaseibacillus paracasei CBA L74 (FM-CBAL74) in preventing pediatric infectious diseases. Rotavirus (RV) is a non-enveloped double-stranded RNA (dsRNA) virus that is the leading cause of acute gastroenteritis-associated morbidity and mortality in early childhood. RV is responsible >500.000 deaths and approximately 2.4 million hospitalization in young children worldwide annually. SARS-CoV-2 is a single-stranded RNA (ssRNA) virus responsible for the Coronavirus disease (COVID-19) pandemic responsible for around 5.6 million deaths and more than 110 million cases reported globally in the last 2 years. Here, we investigated the protective action elicited by FM-CBAL74 against RV and SARS-CoV-2 infection in an in vitro model of human gut epithelium. We demonstrated that pre-incubation with FM-CBAL74 inhibited RV-induced epithelial barrier damage (tight junctions (TJs) and adherent junctions’ protein (AJ), and transepithelial electrical resistance evaluation), reactive oxygen species (ROS) and pro-inflammatory cytokines (IL-6, IL-8, TNF-α) release, through MAP kinases pathway activation. Regarding SARS-CoV-2 infection, we demonstrated that pre- incubation with FM-CBA L74 reduced the number of infected cells, the expression of the host functional receptor angiotensin-converting enzyme-2 (ACE2) and the pro-inflammatory cytokines IL-6, VEGFB, IL-15, IL-1β release. Modulating immune and non-immune protective mechanisms, the postbiotic FM-CBAL74 exerts a protective action against RV and SARS-CoV-2 infection. These findings may open new preventive and therapeutic strategies for these conditions.
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