Capece, Gabriele (2009) Different titanium surfaces influence in vitro the biological behavior of SaOS-2 human osteoblast-like cells. [Tesi di dottorato] (Inedito)

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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: Different titanium surfaces influence in vitro the biological behavior of SaOS-2 human osteoblast-like cells.
Autori:
AutoreEmail
Capece, Gabrielecapecegabriele@tin.it
Data: 30 Novembre 2009
Numero di pagine: 46
Istituzione: Università degli Studi di Napoli Federico II
Dipartimento: Scienze odontostomatologiche e maxillo-facciali
Scuola di dottorato: Medicina clinica e sperimentale
Dottorato: Scienze odontostomatologiche
Ciclo di dottorato: 22
Coordinatore del Corso di dottorato:
nomeemail
Rengo, Sandro[non definito]
Tutor:
nomeemail
Ramaglia, Lucaluca.ramaglia@unina.it
Data: 30 Novembre 2009
Numero di pagine: 46
Parole chiave: dental implants, osteoblasts, cell differentiation, extracellular matrix.
Settori scientifico-disciplinari del MIUR: Area 06 - Scienze mediche > MED/28 - Malattie odontostomatologiche
Depositato il: 01 Dic 2009 12:31
Ultima modifica: 30 Apr 2014 19:40
URI: http://www.fedoa.unina.it/id/eprint/4204
DOI: 10.6092/UNINA/FEDOA/4204

Abstract

Osseointegrated dental implants have been successfully used over the past several years, allowing functional replacement of missing teeth. Surface properties of titanium dental implants seem to affect bone cell response. Implant topography appears to modulate cell growth and differentiation of osteoblasts affecting the bone healing around the implant. Optimal roughness and superficial morphology are still controversial and need to be clearly defined. SaOS-2 is a mature osteoblastic cell line, derived from a human osteosarcoma, that several studies have demonstrated to be a relevant cell model due to its large analogies with immature osteoblast cells and better manageability when compared to primary human cell culture. The aim of the present study was to evaluate in vitro the biological behavior of SaOS-2 cells, cultured on two different titanium surfaces, by investigating morphology, adhesion, proliferation, phenotypical bone expression and extra cellular matrix deposition. Our data demonstrate that a sandblasted-acid-etched surface topography, characterized by pore dimension less than 1 μm, may affect in vitro the differentiation of SaOS-2 cells, producing a better differentiation towards an osteoblastic phenotype, as compared to a smooth surface. It is therefore likely that implant surface properties can modulate in vivo the biological behavior of osteoblasts during bone tissue healing.

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