Different titanium surfaces influence in vitro the biological behavior of SaOS-2 human osteoblast-like cells.

Capece, Gabriele (2009) Different titanium surfaces influence in vitro the biological behavior of SaOS-2 human osteoblast-like cells. [Tesi di dottorato] (Inedito)

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Abstract

Osseointegrated dental implants have been successfully used over the past several years, allowing functional replacement of missing teeth. Surface properties of titanium dental implants seem to affect bone cell response. Implant topography appears to modulate cell growth and differentiation of osteoblasts affecting the bone healing around the implant. Optimal roughness and superficial morphology are still controversial and need to be clearly defined. SaOS-2 is a mature osteoblastic cell line, derived from a human osteosarcoma, that several studies have demonstrated to be a relevant cell model due to its large analogies with immature osteoblast cells and better manageability when compared to primary human cell culture. The aim of the present study was to evaluate in vitro the biological behavior of SaOS-2 cells, cultured on two different titanium surfaces, by investigating morphology, adhesion, proliferation, phenotypical bone expression and extra cellular matrix deposition. Our data demonstrate that a sandblasted-acid-etched surface topography, characterized by pore dimension less than 1 μm, may affect in vitro the differentiation of SaOS-2 cells, producing a better differentiation towards an osteoblastic phenotype, as compared to a smooth surface. It is therefore likely that implant surface properties can modulate in vivo the biological behavior of osteoblasts during bone tissue healing.

Tipologia di documento:Tesi di dottorato
Parole chiave:dental implants, osteoblasts, cell differentiation, extracellular matrix.
Settori scientifico-disciplinari MIUR:Area 06 Scienze mediche > MED/28 MALATTIE ODONTOSTOMATOLOGICHE
Coordinatori della Scuola di dottorato:
Coordinatore del Corso di dottoratoe-mail (se nota)
Rengo, Sandro
Tutor della Scuola di dottorato:
Tutor del Corso di dottoratoe-mail (se nota)
Ramaglia, Lucaluca.ramaglia@unina.it
Stato del full text:Accessibile
Data:30 Novembre 2009
Numero di pagine:46
Istituzione:Università degli Studi di Napoli Federico II
Dipartimento o Struttura:Dipartimento di Scienze Odontostomatologiche e Maxillo-Facciali
Tipo di tesi:Dottorato
Stato dell'Eprint:Inedito
Scuola di dottorato:Scuola di Dottorato in Medicina Clinica e Sperimentale
Denominazione del dottorato:Scienze Odontostomatologiche
Ciclo di dottorato:XXII
Numero di sistema:4204
Depositato il:01 Dicembre 2009 13:31
Ultima modifica:04 Agosto 2010 14:26

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