Capece, Gabriele (2009) Different titanium surfaces influence in vitro the biological behavior of SaOS-2 human osteoblast-like cells. [Tesi di dottorato] (Unpublished)


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Item Type: Tesi di dottorato
Uncontrolled Keywords: dental implants, osteoblasts, cell differentiation, extracellular matrix.
Date Deposited: 01 Dec 2009 12:31
Last Modified: 30 Apr 2014 19:40


Osseointegrated dental implants have been successfully used over the past several years, allowing functional replacement of missing teeth. Surface properties of titanium dental implants seem to affect bone cell response. Implant topography appears to modulate cell growth and differentiation of osteoblasts affecting the bone healing around the implant. Optimal roughness and superficial morphology are still controversial and need to be clearly defined. SaOS-2 is a mature osteoblastic cell line, derived from a human osteosarcoma, that several studies have demonstrated to be a relevant cell model due to its large analogies with immature osteoblast cells and better manageability when compared to primary human cell culture. The aim of the present study was to evaluate in vitro the biological behavior of SaOS-2 cells, cultured on two different titanium surfaces, by investigating morphology, adhesion, proliferation, phenotypical bone expression and extra cellular matrix deposition. Our data demonstrate that a sandblasted-acid-etched surface topography, characterized by pore dimension less than 1 μm, may affect in vitro the differentiation of SaOS-2 cells, producing a better differentiation towards an osteoblastic phenotype, as compared to a smooth surface. It is therefore likely that implant surface properties can modulate in vivo the biological behavior of osteoblasts during bone tissue healing.

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