Carullo, Paola (2010) Physico-chemical characterization of the interaction between thyroid transcription factor 1 (TTF-1) and DNA. [Tesi di dottorato] (Inedito)
Anteprima |
PDF
Carullo_Paola_22.pdf Download (2MB) | Anteprima |
| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Physico-chemical characterization of the interaction between thyroid transcription factor 1 (TTF-1) and DNA |
| Autori: | Autore Email Carullo, Paola paola.carullo@unina.it |
| Data: | 29 Novembre 2010 |
| Numero di pagine: | 96 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Chimica "Paolo Corradini" |
| Scuola di dottorato: | Scienze chimiche |
| Dottorato: | Scienze chimiche |
| Ciclo di dottorato: | 22 |
| Coordinatore del Corso di dottorato: | nome email Previtera, Lucio lucio.previtera@unina.it |
| Tutor: | nome email Del Vecchio, Pompea pompea.delvecchio@unina.it |
| Data: | 29 Novembre 2010 |
| Numero di pagine: | 96 |
| Parole chiave: | thyroid transcription factor 1 (TTF-1); Pax-8 paired-domain; homeodomain; DNA-binding energetics; differential scanning calorimetry; isothermal titration calorimetry; circular dichroism; molecular dynamics |
| Settori scientifico-disciplinari del MIUR: | Area 03 - Scienze chimiche > CHIM/02 - Chimica fisica |
| Depositato il: | 02 Dic 2010 08:00 |
| Ultima modifica: | 30 Apr 2014 19:44 |
| URI: | http://www.fedoa.unina.it/id/eprint/8096 |
| DOI: | 10.6092/UNINA/FEDOA/8096 |
Abstract
The thyroid transcription factor 1 (TTF-1) is a tissue-specific transcription factor involved in the development of thyroid and lung. It is a protein of 371 amino acids that possesses two independent transcriptional activation domains, one located in the N-terminal region of homeodomain and the other in the C-terminal region. The homeodomain is a highly conserved DNA-binding motif that is found in numerous transcription factors throughout a large variety of species from yeasts to human. These gene-specific transcription factors play critical roles in development and adult homeostasis, and therefore, any germline mutations associated with these proteins can lead to a number of congenital abnormalities. Remarkable features of structural and functional conservation have been observed within homeodomain family members. This high degree of conservation between the sequence and structure makes the homeodomain an ideal model for studying protein-DNA interactions and gene regulation. In this PhD thesis, the study on the conformational stability and DNA binding energetics of the rat thyroid transcription factor 1 homeodomain (TTF-1HD) is reported. Furthermore, to gain a molecular-level understanding of the interactions determining the association of TTF-1HD to the target DNA sequence, Some key point mutants (W48, Q50 and Y54) and a deletion mutant (desTTF-1HD), lacking five residues from the N-terminal region have been produced and studied. Since it has been demonstrated that N-terminal region of TTF-1 also interact with Pax-8 (transcription factor belonging to the PAX genes superfamily) in the regulation of thyroid-specific gene expression, the study of the thermal stability and DNA binding energetics of a fusion protein, containing the N-terminal region and the homeodomain of TTF-1 (TTF-1NH2HD), and a recombinant protein containing C-terminal region of Pax-8 (Pax-8 Prd) has been reported.
Downloads
Downloads per month over past year
Actions (login required)
 |
Modifica documento |
