Binazzi, Francesco (2014) HYMENOPTERAN PARASITOIDS: LABORATORY REARING AND EVALUATION OF STORAGE TECHNIQUES. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Resource language: English
Title: HYMENOPTERAN PARASITOIDS: LABORATORY REARING AND EVALUATION OF STORAGE TECHNIQUES
Creators:
Creators
Email
Binazzi, Francesco
francesco.binazzi@entecra.it
Date: 31 March 2014
Number of Pages: 71
Institution: Università degli Studi di Napoli Federico II
Department: Agraria
Scuola di dottorato: Biotecnologie
Dottorato: Insect science and biotechnology
Ciclo di dottorato: 26
Coordinatore del Corso di dottorato:
nome
email
Pennacchio, Francesco
f.pennacchio@unina.it
Tutor:
nome
email
Roversi, Pio Federico
UNSPECIFIED
Date: 31 March 2014
Number of Pages: 71
Keywords: biological control, parasiotids, cold storage, cooling rate, heating rate
Settori scientifico-disciplinari del MIUR: Area 07 - Scienze agrarie e veterinarie > AGR/11 - Entomologia generale e applicata
Aree tematiche (7° programma Quadro): BIOTECNOLOGIE, PRODOTTI ALIMENTARI E AGRICOLTURA > Produzione sostenibile e gestione delle risorse biologiche della terra, della foresta e dell'ambiente acquatico
Date Deposited: 08 Apr 2014 14:24
Last Modified: 15 Jul 2015 01:02
URI: http://www.fedoa.unina.it/id/eprint/9994

Collection description

The present work focused on O. pityocampae (Mercet) (Hymenoptera Encyrtidae), an indigenous and polyphagous egg-parasitoid that might play a role in biological control programs against Thaumetopoea pityocampa (Den. & Shiff.) (Lepidoptera Notodontidae). Therefore biological parameters of this encyrtid reared on alternative host eggs were analyzed by testing its behavior and reproductive parameters. Time between oviposition and progeny emergence, sex ratio of offspring, parasitization rate and offspring production were recorded. Thereafter, in a second set of experiments, the stages of O. pityocampae juveniles developing within eggs of Nezara viridula (L.) (Heteroptera Pentaomidae) were also identified by morphological analysis and measurements. Egg-to-adult temperature-dependent development of O. pityocampae within eggs of the alternative host Graphosoma lineatum (L.) (Heteroptera Pentaomidae) was also investigated in order to determine development times. Moreover, because parasitoid development within host eggs is often influenced by host egg age, trials were carried out to determine the capacity of this encyrtid to accept and exploit eggs from different age classes. Since maintenance of living organisms is particularly useful in mass rearing programs, the second part of the project was aimed at evaluating cold storage potential of O. pityocampae for long periods. For this reason cold tolerance of this parasitoid was evaluated by storing different juvenile stages within eggs of G. lineatum at several low temperatures for 10 days. The effect of cooling and heating rates on the survival of stored immatures was also assessed for each thermal treatment.

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