Trotta, Roberta (2011) Insight into macromolecular interactions involving nucleic acids: a study of the interaction of G-quadruplexes with pharmacologically active molecules and investigations of the RNA binding properties of LARP4 protein. [Tesi di dottorato] (Inedito)
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Testo
Trotta_Roberta_24.pdf Download (10MB) | Anteprima |
| Tipologia del documento: | Tesi di dottorato | ||||
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| Lingua: | English | ||||
| Titolo: | Insight into macromolecular interactions involving nucleic acids: a study of the interaction of G-quadruplexes with pharmacologically active molecules and investigations of the RNA binding properties of LARP4 protein. | ||||
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| Data: | 30 Novembre 2011 | ||||
| Numero di pagine: | 198 | ||||
| Istituzione: | Università degli Studi di Napoli Federico II | ||||
| Dipartimento: | Chimica delle sostanze naturali | ||||
| Scuola di dottorato: | Scienze farmaceutiche | ||||
| Dottorato: | Scienza del farmaco | ||||
| Ciclo di dottorato: | 24 | ||||
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| Data: | 30 Novembre 2011 | ||||
| Numero di pagine: | 198 | ||||
| Parole chiave: | G-quadruplex;NMR;LARP4 protein | ||||
| Settori scientifico-disciplinari del MIUR: | Area 03 - Scienze chimiche > CHIM/06 - Chimica organica Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 03 - Scienze chimiche > CHIM/08 - Chimica farmaceutica |
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| Depositato il: | 06 Dic 2011 11:35 | ||||
| Ultima modifica: | 15 Lug 2015 01:00 | ||||
| URI: | http://www.fedoa.unina.it/id/eprint/8950 |
Abstract
This dissertation can be divided in two parts. In the first part, my efforts have been focused on the study of the Gquadruplex structures. In chapter one, I describe the unusual arrangement of the DNA to form the G-quadruplex structures and their biological role, underlying the reason why in the last period a lot of research groups focused their studies on this topic. In chapter two, after a brief introduction of the first molecules capable to bind the G-quadruplex (Distamicyn A and its derivates), I describe the new organic molecule capable to interact with the DNA quadruplex, which I have discovered applying some innovative approaches during my PhD. In the second part of this thesis I introduce the research conducted in the laboratory of Dr. Maria R. Conte at the King’s College of London. In this stimulating environment I started studying the structure of the protein LARP4 and its interaction with a poly (A) RNA using the NMR. In the course of my research I have learnt the techniques allowing to do the transformation, the expression and the purification of the protein. A summary presentation of the techniques I have applied is reported in the second part in chapter five.
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