Romano, Miriam (2024) Directed evolution of human Adenoviruses to improve vectored vaccines properties. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Directed evolution of human Adenoviruses to improve vectored vaccines properties |
| Autori: | Autore Email Romano, Miriam miry1502@hotmail.it |
| Data: | 11 Marzo 2024 |
| Numero di pagine: | 76 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Medicina Molecolare e Biotecnologie Mediche |
| Dottorato: | Medicina molecolare e biotecnologie mediche |
| Ciclo di dottorato: | 36 |
| Coordinatore del Corso di dottorato: | nome email Santoro, Massimo masantor@unina.it |
| Tutor: | nome email Nicosia, Alfredo [non definito] |
| Data: | 11 Marzo 2024 |
| Numero di pagine: | 76 |
| Parole chiave: | Adenovirus vector libraries, diversity, NGS analysis, selection |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare |
| Depositato il: | 20 Mar 2024 14:19 |
| Ultima modifica: | 12 Ago 2026 05:33 |
| URI: | https://www.fedoa.unina.it/id/eprint/15459 |
Abstract
Replication incompetent Adeno (Ad)-based vectors are able to induce potent adaptive immune responses in animal models and humans, being of interest for both infectious diseases and cancer therapy or prevention. Systematic approaches allowing the generation of high diversity Ad variants libraries represent invaluable tools for the selection of novel vaccine vectors with improved properties. We have set up a procedure allowing the generation of libraries of Adenoviral vectors genomic variants with an unprecedented high diversity. Six adenoviral genome libraries were generated with mutations in single HVRs. Overall, more than 10⁶ genomic variants were obtained. This complex repertoire of Ad vectors was then successfully rescued. The vector libraries obtained were subjected to different selective pressures, leading to identification of variants enriched in different conditions, potentially associated to improved growth properties or reduced recognition by anti-Ad antibodies present in humans.
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