Magliacane Trotta, Sara (2023) Cancer-derived exosomal-Alu RNA promotes colorectal cancer progression by inducing epithelial-to-mesenchymal transition through NLRP3 inflammasome activation. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Cancer-derived exosomal-Alu RNA promotes colorectal cancer progression by inducing epithelial-to-mesenchymal transition through NLRP3 inflammasome activation |
| Autori: | Autore Email Magliacane Trotta, Sara sara.maglicanetrotta@unina.it |
| Data: | 13 Dicembre 2023 |
| Numero di pagine: | 78 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Biologia |
| Dottorato: | Biologia |
| Ciclo di dottorato: | 36 |
| Coordinatore del Corso di dottorato: | nome email Esposito, Sergio sergio.esposito@unina.it |
| Tutor: | nome email Tarallo, Valeria [non definito] De Falco, Sandro [non definito] |
| Data: | 13 Dicembre 2023 |
| Numero di pagine: | 78 |
| Parole chiave: | Alu RNA, colorectal cancer, exosome, NLRP3 inflammasome, EMT |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 05 - Scienze biologiche > BIO/13 - Biologia applicata |
| Depositato il: | 03 Gen 2024 17:59 |
| Ultima modifica: | 12 Ago 2026 05:36 |
| URI: | https://www.fedoa.unina.it/id/eprint/15619 |
Abstract
Inflammation plays a crucial role in cancer progression, but the relevance of the NLRP3 inflammasome remains unclear. Alu RNA is the first endogenous nucleic acid identified to activate the NLRP3 inflammasome. Here, we show that Alu RNA can induce epithelial-to-mesenchymal transition (EMT) through NLRP3 inflammasome activation and releasing IL-1b in colorectal cancer (CRC) cells. Alu RNA is stored, transported and transferred to CRC cells by exosomes. Exosomal-Alu RNA promotes tumorigenesis by inducing invasion, metastasis and EMT through NLRP3 inflammasome activation. Corroborating this data, we found that the significantly increased expression of Alu RNA correlates with the induction of NLRP3 priming in human CRC patients. Furthermore, the expression level of Alu RNA from circulating exosomes correlate with CRC progression in preclinical model. These findings reveal the direct involvement of Alu RNAs in cancer pathogenesis and their presence in CRC cell-derived exosomes could be used as non-invasive diagnostic biomarker.
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