Capasso, Mario (2025) THE EFFECT OF HEAT-TREATED AKKERMANSIA MUNICIPHILA MucT ON HEPATIC CELL MODEL OF HEPATOCELLULAR CARCINOMA. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | THE EFFECT OF HEAT-TREATED AKKERMANSIA MUNICIPHILA MucT ON HEPATIC CELL MODEL OF HEPATOCELLULAR CARCINOMA |
| Autori: | Autore Email Capasso, Mario mario.capa05@gmail.com |
| Data: | 9 Dicembre 2025 |
| Numero di pagine: | 31 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Medicina Clinica e Chirurgia |
| Dottorato: | Terapie avanzate biomediche e chirurgiche |
| Ciclo di dottorato: | 38 |
| Coordinatore del Corso di dottorato: | nome email Settembre, Carmine carmine.settembre@unina.it |
| Tutor: | nome email Morisco, Filomena [non definito] |
| Data: | 9 Dicembre 2025 |
| Numero di pagine: | 31 |
| Parole chiave: | Akkermansia muciniphila, HCC, apoptosis, autophagy |
| Settori scientifico-disciplinari del MIUR: | Area 06 - Scienze mediche > MED/12 - Gastroenterologia |
| Depositato il: | 21 Gen 2026 21:53 |
| Ultima modifica: | 12 Ago 2026 05:37 |
| URI: | https://www.fedoa.unina.it/id/eprint/16139 |
Abstract
Background: Dysbiosis, through the gut–liver axis, can influence the pathogenesis of hepatocellular carcinoma (HCC). Recently, Akkermansia muciniphila has been shown to play a significant protective role in the development of HCC. Aim: To assess the effects of heat-treated A. muciniphila MucT on cell viability and autophagy/apoptosis signaling in HCC in vitro models. Methods: Murine Hepa 1-6 and NMuLi and human HepG2 cells were exposed to A. muciniphila (10³ or 10 CFU) for 12–72 h after which cell viability was determined by MTT and samples were collected to assess the expression of the proteins of interest by Western blot (p-mTOR/mTOR, p70S6K, S6, Beclin-1, p62, Caspase-3). Results: A. muciniphila at 10³ CFU significantly reduced viability in Hepa 1-6 (24-48 h) and HepG2 (12–48 h); NMuLi showed an early drop at 12 h that faded by 48 h. In Hepa 1-6, p-mTOR and downstream effectors (p70S6K, S6) decreased, consistent with mTORC1 inhibition and autophagy engagement, despite a concurrent Beclin-1 reduction. While no changes were observed in mTOR-pathway markers and Beclin-1, an increase in Caspase-3 expression was found in NMuLi cells, suggesting apoptosis as the main driver of reduced viability. In HepG2, p-mTOR/mTOR rose slightly while p-p70/p70 and p-S6/S6 decreased. Conclusions: Heat-treated A. muciniphila MucT directly modulates hepatic tumor cell viability and key nodes of pathways related to autophagy/apoptosis, in a cell line specific manner. These findings support the potential usefulness of A. muciniphila as an adjuvant strategy to improve treatment response in HCC patients.
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