Mileo, Aldo (2025) Impact of Endocrine Disrupting Chemicals on Human Prostate Cells Physiology. [Tesi di dottorato]

	Documento PDF Impact_of_Endocrine_Disrupting_Chemicals_on_Human_Prostate_Cellls_Physiology.pdf Visibile a [TBR] Amministratori dell'archivio Download (35MB) | Richiedi una copia
	Documento PDF EMBARGO_impact_of_Endocrine_Disrupting_Chemicals_on_Human_Prostate_Cells_Physiology.pdf Visibile a [TBR] Amministratori dell'archivio Download (30MB) | Richiedi una copia

Abstract

One of the most widely used materials in modern times is plastic, used to produce objects for domestic, food and industrial purposes. To make it more attractive on the market and to improve its chemical and physical qualities, chemical additives are often used. Since these substances are not covalently bound to the polymer matrix of plastics, they can easily leach into the environment, bioaccumulating in the water, soil and air matrices. Most of these chemical additives belong to the class of endocrine disrupting chemicals (EDCs), defined as exogenous substances (or mixture of substances) that disrupt the homeostatic balance of the endocrine system of living beings (Zoeller et al., 2012; Gore et al., 2015). Continuous exposure to these types of contaminants, albeit at low concentrations, leads to severe consequences in reproduction: infertility, reduced fertility, miscarriages and abnormalities in both the male and female reproductive tracts are the most frequent disorders following chronic exposure to EDCs (McKinlay et al., 2008; World Health Organisation, 2013). Although their presence in the environment raises much concern in the scientific community, there is little scientific evidence on the cocktail effect and the ability of EDCs to act additively, antagonistically or synergistically with each other or with natural hormones present in the individual. Keeping in in mind these knowledges, the aim of this research was to deepen our in vitro knowledge of EDCs both under physiological conditions, by studying the effect of Dibutylphthalate and Nonylphenol, in a mixture with endogenous hormones (Testosterone and 17-β-oestradiol), and assessing their possible additive effect, antagonist or synergistic effect on non-cancerous prostate cells (PNT1A) in order to assess their possible involvement in carcinogenesis, and under pathological conditions, by studying the effect of Bisphenol A on cancerous prostate cells (DU145 and LNCAP) by assessing its role at a stage of disease already confirmed in the prostate gland.

Downloads

Actions (login required)

Modifica documento