Evangelista, Adriana Rita (2025) Development of a foodomics platform for monitoring the basil transformation process for the food industry. [Tesi di dottorato]

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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: Development of a foodomics platform for monitoring the basil transformation process for the food industry
Autori:
Autore
Email
Evangelista, Adriana Rita
adrianarita.evangelista@unina.it
Data: Febbraio 2025
Numero di pagine: 142
Istituzione: Università degli Studi di Napoli Federico II
Dottorato: Food Science
Ciclo di dottorato: 38
Coordinatore del Corso di dottorato:
nome
email
Barone, Amalia
Amalia.barone@unina.it
Tutor:
nome
email
Andolfo, Giuseppe
[non definito]
Nitride, Chiara
[non definito]
Data: Febbraio 2025
Numero di pagine: 142
Parole chiave: Multi-omics integration, Eugenol Synthase (EGS), Ocimum basilicum
Settori scientifico-disciplinari del MIUR: Area 07 - Scienze agrarie e veterinarie > AGR/07 - Genetica agraria
Area 07 - Scienze agrarie e veterinarie > AGR/15 - Scienze e tecnologie alimentari
Informazioni aggiuntive: 38°ciclo Food Science
Depositato il: 29 Dic 2025 22:15
Ultima modifica: 12 Ago 2026 05:39
URI: https://www.fedoa.unina.it/id/eprint/17116

Abstract

This thesis develops an integrated omics framework to investigate both the molecular architecture underlying aroma formation in Ocimum basilicum (cv. Eleonora) and the sustainable valorisation of by-products derived from aromatic plants. In the first part of the work, a multi-omic platform was established to characterise basil across five developmental stages. Transcriptomic analysis revealed more than 65,000 expressed genes and extensive developmental remodelling, while phylogenetic and functional assessments identified new Eugenol Synthase (ObEGS) isoforms, displaying clade-specific and stage-dependent expression profiles. The integration of transcriptomic, proteomic, and targeted metabolomic datasets enabled a detailed reconstruction of the phenolic and volatile pathways that shape the aromatic phenotype of basil. The second part of the thesis extends this omics-based approach to by-products generated from the processing of aromatic herbs, including dill, basil, sage, coriander, and rosemary. High-resolution mass spectrometry revealed pronounced species-specific differences in polyphenols, proteins, volatile constituents, and in silico predicted bioactive peptides with potential antioxidant and ACE-inhibitory activities. Collectively, these findings provide a comprehensive, multidimensional application of omics sciences: elucidating the molecular determinants of aroma and quality in basil while advancing analytical strategies for converting aromatic plant by-products into value-added resources. The platform developed herein offers a transferable model for other aromatic species. It contributes to the progression of integrated and predictive foodomics aimed at innovation, traceability, and sustainability within the agri-food sector.

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