Longobardi, Valentina (2015) Innovative strategies to improve fertility of buffalo semen. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: Innovative strategies to improve fertility of buffalo semen
Creators:
CreatorsEmail
Longobardi, Valentinalongobardivalentina@gmail.com
Date: 28 March 2015
Number of Pages: 139
Institution: Università degli Studi di Napoli Federico II
Department: Medicina Molecolare e Biotecnologie Mediche
Scuola di dottorato: Scienze veterinarie per la produzione e la sanità
Dottorato: Produzione e sanità degli alimenti di origine animale
Ciclo di dottorato: 27
Coordinatore del Corso di dottorato:
nomeemail
Cortesi, Maria Luisacortesi@unina.it
Tutor:
nomeemail
Campanile, GiuseppeUNSPECIFIED
Gasparrini, BiancaUNSPECIFIED
Date: 28 March 2015
Number of Pages: 139
Uncontrolled Keywords: buffalo semen; fertility; cryocapacitation
Settori scientifico-disciplinari del MIUR: Area 07 - Scienze agrarie e veterinarie > AGR/19 - Zootecnica speciale
Date Deposited: 14 Apr 2015 09:12
Last Modified: 29 Sep 2015 08:19
URI: http://www.fedoa.unina.it/id/eprint/10169
DOI: 10.6092/UNINA/FEDOA/10169

Abstract

The improvement of fertility is an important contribution that applied scientific research can provide to buffalo breeding, especially in productive areas like the Campania region. Many studies have been conducted to improve the management of the female reproductive system, while insufficient are the information about the effect of the male on the reproductive efficiency in this species. Buffalo is a photoperiodic animal that tends to increase the reproductive activity during periods when daylight hours decrease. In particular, in the male, it is manifested by a reduction of libido when daylight hours increase. This causes a reduction in the number of jumps and a worsening of the qualitative characteristics of the semen produced . Also, the cryopreservation process influences the quality of the semen causing premature sperm capacitation hence, reducing its longevity in the female reproductive tract. At present, for cryopreservation of semen, standard protocols are used equally for all species, not considering the significant interspecific differences that exist. The aim of the research was, therefore, to evaluate the effect of season on buffalo frozen-thawed semen and to improve its quality by analyzing the effects of the addition of antioxidants such as resveratrol at various concentrations (0.5, 1, 10 and 50 uM), carnitine (2.5 and 7.5 mM ) and sterols, such as cholesterol (1.5 and 3 mg /ml), to the standard freezing extenders. The addition of antioxidants and / or sterol has stabilized the sperm membrane, reducing the state of capacitation, improving motility and consequently semen quality. The effect of the photoperiod was evident with a better semen quality during autumn. Based on these results was also evaluated fertilizing capacity both in vivo (resveratrol and cholesterol) and in vitro (resveratrol and carnitine)selecting concentrations that showed a more marked effect on the stabilization of the sperm membrane. The results obtained suggest that the premature capacitation after cryopreservation of spermatozoa is reduced during the favorable season and that the addition of antioxidants and / or sterols has a positive effect on buffalo semen quality and consequently, on in vitro and in vivo fertility.

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