Di Luise, Nunzia
(2015)
Molecularly imprinted polymers with assistant recognition biomolecule for protein detection.
[Tesi di dottorato]
| Tipologia del documento: |
Tesi di dottorato
|
| Lingua: |
English |
| Titolo: |
Molecularly imprinted polymers with assistant recognition biomolecule for protein detection |
| Autori: |
| Autore | Email |
|---|
| Di Luise, Nunzia | nunzia.diluise@googlemail.com |
|
| Data: |
30 Marzo 2015 |
| Numero di pagine: |
117 |
| Istituzione: |
Università degli Studi di Napoli Federico II |
| Dipartimento: |
Ingegneria Chimica, dei Materiali e della Produzione Industriale |
| Scuola di dottorato: |
Ingegneria industriale |
| Dottorato: |
Ingegneria dei materiali e delle strutture |
| Ciclo di dottorato: |
27 |
| Coordinatore del Corso di dottorato: |
| nome | email |
|---|
| Mensitieri, Giuseppe | mensitie@unina.it |
|
| Tutor: |
| nome | email |
|---|
| Causa, Filippo | [non definito] | | Netti, Paolo Antonio | [non definito] | | Battista, Edmondo | [non definito] |
|
| Data: |
30 Marzo 2015 |
| Numero di pagine: |
117 |
| Parole chiave: |
polimeri a stampo molecolare; riconoscimento molecolare; biosensore |
| Settori scientifico-disciplinari del MIUR: |
Area 09 - Ingegneria industriale e dell'informazione > ING-IND/22 - Scienza e tecnologia dei materiali |
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| Informazioni aggiuntive: |
Tesi svolta presso l'Istituto Italiano di Tecnologia (IIT), Napoli |
| Depositato il: |
12 Apr 2015 01:19 |
| Ultima modifica: |
29 Apr 2016 01:00 |
| URI: |
http://www.fedoa.unina.it/id/eprint/10279 |
| DOI: |
10.6092/UNINA/FEDOA/10279 |
Abstract
Molecularly imprinted polymers are ideal alternatives to natural recognition elements for a variety of reasons, including facile synthesis, greater chemical and long term stability, and reusability. One of the most challenging tasks in developing such polymers is provide them of a signal transduction capability, enabling to respond to a specific binding event. In this thesis, protein-imprinted polymers, capable of specific transduction of binding event into a fluorescence change were prepared using an assistant-peptide bearing an environment-sensitive fluorophore. The preparation has included the synthesis of the environment-sensitive peptide and subsequent incorporation into the polymer network through the imprinting process. Binding studies proved that MIP-SA-allyl-peptide has large absorption capacity and good affinity and selectivity toward BSA when compared with pure MIP. The greater binding properties of MIP-SA-allyl-peptide were found to derive from the assistant-peptide that suitably oriented into the cavity, acts as binding site in cooperation with the imprinted cavity. Furthermore, transduction signaling studies proved that MIP-SA-allyl-dansyl-peptide is able to detect and report the protein binding into a precise detection range. The proposed fluorescent-imprinted polymer provides a new and general strategy for protein-sensing platforms and opens up to the field of biosensors.
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