Di Luise, Nunzia (2015) Molecularly imprinted polymers with assistant recognition biomolecule for protein detection. [Tesi di dottorato]

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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: Molecularly imprinted polymers with assistant recognition biomolecule for protein detection
Autori:
AutoreEmail
Di Luise, Nunzianunzia.diluise@googlemail.com
Data: 30 Marzo 2015
Numero di pagine: 117
Istituzione: Università degli Studi di Napoli Federico II
Dipartimento: Ingegneria Chimica, dei Materiali e della Produzione Industriale
Scuola di dottorato: Ingegneria industriale
Dottorato: Ingegneria dei materiali e delle strutture
Ciclo di dottorato: 27
Coordinatore del Corso di dottorato:
nomeemail
Mensitieri, Giuseppemensitie@unina.it
Tutor:
nomeemail
Causa, Filippo[non definito]
Netti, Paolo Antonio[non definito]
Battista, Edmondo[non definito]
Data: 30 Marzo 2015
Numero di pagine: 117
Parole chiave: polimeri a stampo molecolare; riconoscimento molecolare; biosensore
Settori scientifico-disciplinari del MIUR: Area 09 - Ingegneria industriale e dell'informazione > ING-IND/22 - Scienza e tecnologia dei materiali
Informazioni aggiuntive: Tesi svolta presso l'Istituto Italiano di Tecnologia (IIT), Napoli
Depositato il: 12 Apr 2015 01:19
Ultima modifica: 29 Apr 2016 01:00
URI: http://www.fedoa.unina.it/id/eprint/10279
DOI: 10.6092/UNINA/FEDOA/10279

Abstract

Molecularly imprinted polymers are ideal alternatives to natural recognition elements for a variety of reasons, including facile synthesis, greater chemical and long term stability, and reusability. One of the most challenging tasks in developing such polymers is provide them of a signal transduction capability, enabling to respond to a specific binding event. In this thesis, protein-imprinted polymers, capable of specific transduction of binding event into a fluorescence change were prepared using an assistant-peptide bearing an environment-sensitive fluorophore. The preparation has included the synthesis of the environment-sensitive peptide and subsequent incorporation into the polymer network through the imprinting process. Binding studies proved that MIP-SA-allyl-peptide has large absorption capacity and good affinity and selectivity toward BSA when compared with pure MIP. The greater binding properties of MIP-SA-allyl-peptide were found to derive from the assistant-peptide that suitably oriented into the cavity, acts as binding site in cooperation with the imprinted cavity. Furthermore, transduction signaling studies proved that MIP-SA-allyl-dansyl-peptide is able to detect and report the protein binding into a precise detection range. The proposed fluorescent-imprinted polymer provides a new and general strategy for protein-sensing platforms and opens up to the field of biosensors.

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