Di Luise, Nunzia (2015) Molecularly imprinted polymers with assistant recognition biomolecule for protein detection. [Tesi di dottorato]


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Item Type: Tesi di dottorato
Resource language: English
Title: Molecularly imprinted polymers with assistant recognition biomolecule for protein detection
Di Luise, Nunzianunzia.diluise@googlemail.com
Date: 30 March 2015
Number of Pages: 117
Institution: Università degli Studi di Napoli Federico II
Department: Ingegneria Chimica, dei Materiali e della Produzione Industriale
Scuola di dottorato: Ingegneria industriale
Dottorato: Ingegneria dei materiali e delle strutture
Ciclo di dottorato: 27
Coordinatore del Corso di dottorato:
Mensitieri, Giuseppemensitie@unina.it
Netti, Paolo AntonioUNSPECIFIED
Battista, EdmondoUNSPECIFIED
Date: 30 March 2015
Number of Pages: 117
Keywords: polimeri a stampo molecolare; riconoscimento molecolare; biosensore
Settori scientifico-disciplinari del MIUR: Area 09 - Ingegneria industriale e dell'informazione > ING-IND/22 - Scienza e tecnologia dei materiali
Additional information: Tesi svolta presso l'Istituto Italiano di Tecnologia (IIT), Napoli
Date Deposited: 12 Apr 2015 01:19
Last Modified: 29 Apr 2016 01:00
URI: http://www.fedoa.unina.it/id/eprint/10279
DOI: 10.6092/UNINA/FEDOA/10279

Collection description

Molecularly imprinted polymers are ideal alternatives to natural recognition elements for a variety of reasons, including facile synthesis, greater chemical and long term stability, and reusability. One of the most challenging tasks in developing such polymers is provide them of a signal transduction capability, enabling to respond to a specific binding event. In this thesis, protein-imprinted polymers, capable of specific transduction of binding event into a fluorescence change were prepared using an assistant-peptide bearing an environment-sensitive fluorophore. The preparation has included the synthesis of the environment-sensitive peptide and subsequent incorporation into the polymer network through the imprinting process. Binding studies proved that MIP-SA-allyl-peptide has large absorption capacity and good affinity and selectivity toward BSA when compared with pure MIP. The greater binding properties of MIP-SA-allyl-peptide were found to derive from the assistant-peptide that suitably oriented into the cavity, acts as binding site in cooperation with the imprinted cavity. Furthermore, transduction signaling studies proved that MIP-SA-allyl-dansyl-peptide is able to detect and report the protein binding into a precise detection range. The proposed fluorescent-imprinted polymer provides a new and general strategy for protein-sensing platforms and opens up to the field of biosensors.


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