Di Luise, Nunzia (2015) Molecularly imprinted polymers with assistant recognition biomolecule for protein detection. [Tesi di dottorato]
Preview |
Text
di_luise_nunzia_27.pdf Download (3MB) | Preview |
| Item Type: | Tesi di dottorato |
|---|---|
| Resource language: | English |
| Title: | Molecularly imprinted polymers with assistant recognition biomolecule for protein detection |
| Creators: | Creators Email Di Luise, Nunzia nunzia.diluise@googlemail.com |
| Date: | 30 March 2015 |
| Number of Pages: | 117 |
| Institution: | Università degli Studi di Napoli Federico II |
| Department: | Ingegneria Chimica, dei Materiali e della Produzione Industriale |
| Scuola di dottorato: | Ingegneria industriale |
| Dottorato: | Ingegneria dei materiali e delle strutture |
| Ciclo di dottorato: | 27 |
| Coordinatore del Corso di dottorato: | nome email Mensitieri, Giuseppe mensitie@unina.it |
| Tutor: | nome email Causa, Filippo UNSPECIFIED Netti, Paolo Antonio UNSPECIFIED Battista, Edmondo UNSPECIFIED |
| Date: | 30 March 2015 |
| Number of Pages: | 117 |
| Keywords: | polimeri a stampo molecolare; riconoscimento molecolare; biosensore |
| Settori scientifico-disciplinari del MIUR: | Area 09 - Ingegneria industriale e dell'informazione > ING-IND/22 - Scienza e tecnologia dei materiali |
| Additional information: | Tesi svolta presso l'Istituto Italiano di Tecnologia (IIT), Napoli |
| Date Deposited: | 12 Apr 2015 01:19 |
| Last Modified: | 29 Apr 2016 01:00 |
| URI: | http://www.fedoa.unina.it/id/eprint/10279 |
| DOI: | 10.6092/UNINA/FEDOA/10279 |
Collection description
Molecularly imprinted polymers are ideal alternatives to natural recognition elements for a variety of reasons, including facile synthesis, greater chemical and long term stability, and reusability. One of the most challenging tasks in developing such polymers is provide them of a signal transduction capability, enabling to respond to a specific binding event. In this thesis, protein-imprinted polymers, capable of specific transduction of binding event into a fluorescence change were prepared using an assistant-peptide bearing an environment-sensitive fluorophore. The preparation has included the synthesis of the environment-sensitive peptide and subsequent incorporation into the polymer network through the imprinting process. Binding studies proved that MIP-SA-allyl-peptide has large absorption capacity and good affinity and selectivity toward BSA when compared with pure MIP. The greater binding properties of MIP-SA-allyl-peptide were found to derive from the assistant-peptide that suitably oriented into the cavity, acts as binding site in cooperation with the imprinted cavity. Furthermore, transduction signaling studies proved that MIP-SA-allyl-dansyl-peptide is able to detect and report the protein binding into a precise detection range. The proposed fluorescent-imprinted polymer provides a new and general strategy for protein-sensing platforms and opens up to the field of biosensors.
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |
