Crespo Pardo, David (2015) DNA molecular markers in mosses and their use for the characterization of a Sphagnum palustre clone, for its exploitation as a passive contaminant sensor. [Tesi di dottorato]

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Item Type:	Tesi di dottorato
Resource language:	English
Title:	DNA molecular markers in mosses and their use for the characterization of a Sphagnum palustre clone, for its exploitation as a passive contaminant sensor
Creators:	Creators Email Crespo Pardo, David david.crespo@hotmail.es
Date:	2015
Number of Pages:	71
Institution:	Università degli Studi di Napoli Federico II
Department:	Biologia
Scuola di dottorato:	Scienze biologiche
Dottorato:	Biologia avanzata
Ciclo di dottorato:	27
Coordinatore del Corso di dottorato:	nome email Gaudio, Luciano gaudio@unina.it
Tutor:	nome email Giordano, Simonetta UNSPECIFIED
Date:	2015
Number of Pages:	71
Keywords:	Sphagnum palustre, molecular characterization, molecular markers.
Settori scientifico-disciplinari del MIUR:	Area 05 - Scienze biologiche > BIO/04 - Fisiologia vegetale Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Date Deposited:	07 Apr 2015 13:47
Last Modified:	30 Sep 2015 16:45
URI:	http://www.fedoa.unina.it/id/eprint/10358
DOI:	10.6092/UNINA/FEDOA/10358

Collection description

The control of the air quality is often assessed by the use of terrestrial mosses. However, due to the lack of standardization in the used techniques, the comparison between the available studies is difficult. My PhD thesis was related to the European project FP7-Mossclone. The aim of this project is the development of a standardized method for biomonitoring the level of atmospheric pollution through the use of a devitalized moss clone of Sphagnum palustre, using the moss bags technique. My particular task was to perform the molecular characterization with DNA molecular markers of the clone, in order to label it for a subsequent patent development. First a bibliographical research of the application of DNA molecular markers in moss studies was performed and published. Furthermore, I have applied the acquired knowledge of the molecular techniques into a particular case: the Hypnum cupressiforme complex, focalizing the study in the distinction between Hypnum lacunosum and Hypnum cupressiforme using the intron-exon splice junction (ISJ) marker. I present as well some experiments that will help to optimize some cell culture techniques towards biotechnology and biomonitoring. Particularly, I studied some culture techniques in the aquatic moss Rhynchostegium riparioides, I studied some aspects concerning the protoplast isolation protocol in Sphagnum palustre and I have identified and sequenced a Sphagnum palustre sequence from the actin family.

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