Pellegrino, Grazia (2015) The adipokine apelin-13 induces expression of prothrombotic tissue factor. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: The adipokine apelin-13 induces expression of prothrombotic tissue factor
Creators:
CreatorsEmail
Pellegrino, Graziastellapeg18@hotmail.it
Date: 31 March 2015
Number of Pages: 37
Institution: Università degli Studi di Napoli Federico II
Department: Scienze Biomediche Avanzate
Scuola di dottorato: Medicina clinica e sperimentale
Dottorato: Fisiopatologia clinica e medicina sperimentale
Ciclo di dottorato: 27
Coordinatore del Corso di dottorato:
nomeemail
Marone, GianniUNSPECIFIED
Tutor:
nomeemail
Cirillo, PlinioUNSPECIFIED
Date: 31 March 2015
Number of Pages: 37
Uncontrolled Keywords: Adipokines, atherothrombosis, tissue factor
Settori scientifico-disciplinari del MIUR: Area 06 - Scienze mediche > MED/11 - Malattie dell'apparato cardiovascolare
Aree tematiche (7° programma Quadro): SALUTE e TUTELA DEL CONSUMATORE > Biotecnologie, strumenti e tecnologie generiche per la salute umana
Date Deposited: 08 Apr 2015 11:10
Last Modified: 07 Oct 2015 10:28
URI: http://www.fedoa.unina.it/id/eprint/10395
DOI: 10.6092/UNINA/FEDOA/10395

Abstract

Adipocytes are cells able to produce and secrete several active substances (adipokines) with direct effects on vascular cells. Apelin, one of the most recently identified adipokines has been studied in cardiovascular system physiology in regard to vessel vasodilation and myocardial contraction, but it has not yet completely characterised for its pathophysiological role in cardiovascular disease and especially in acute coronary syndromes (ACS). Several studies have indicated that tissue factor (TF) plays a pivotal role in the pathophysiology of ACS by triggering the formation of intracoronary thrombi following endothelial injury. This study investigates the effects of apelin 12 and apelin 13 on TF in human umbilical endothelial cells (HUVECs) and monocytes. Cells were stimulated with increasing concentrations of apelin 12 or apelin 13 and then processed to evaluate TF-mRNA levels by real-time PCR as well as TF expression/activity by FACS analysis and pro-coagulant activity. Finally, a potential molecular pathway involved in modulating this phenomenon was investigated. We demonstrate that apelin 13 but not apelin 12 induces transcription of mRNA for TF. In addition, we show that this adipokine promotes surface expression of TF that is functionally active. Apelin 13 effects on TF appear modulated by the activation of the G-protein-transcription factor nuclear factor (NF)k�B axis since G-protein inhibitors suppressed NF-kB mediated TF expression. Data of the present study, although in vitro, indicate that apelin-13, induces a procoagulant phenotype in HUVECs and monocytes by promoting TF expression. These observations support the hypothesis that this adipokine might play a relevant role as an active partaker in athero-thrombotic disease.

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