Pezone, Antonio
(2015)
Local DNA oxidation and DNA methylation
define chromatin loops and drive
the transcription cycles induced by estrogen.
[Tesi di dottorato]
| Tipologia del documento: |
Tesi di dottorato
|
| Lingua: |
English |
| Titolo: |
Local DNA oxidation and DNA methylation
define chromatin loops and drive
the transcription cycles induced by estrogen |
| Autori: |
| Autore | Email |
|---|
| Pezone, Antonio | antoniopezone@gmail.com |
|
| Data: |
31 Marzo 2015 |
| Numero di pagine: |
63 |
| Istituzione: |
Università degli Studi di Napoli Federico II |
| Dipartimento: |
Medicina Molecolare e Biotecnologie Mediche |
| Scuola di dottorato: |
Medicina molecolare |
| Dottorato: |
Patologia e fisiopatologia molecolare |
| Ciclo di dottorato: |
27 |
| Coordinatore del Corso di dottorato: |
| nome | email |
|---|
| Avvedimento, Vittorio Enrico | avvedim@unina.it |
|
| Tutor: |
| nome | email |
|---|
| Avvedimento, Vittorio Enrico | [non definito] |
|
| Data: |
31 Marzo 2015 |
| Numero di pagine: |
63 |
| Parole chiave: |
DNA Oxidation, DNA Hydroxymethylation, Transcription |
| Settori scientifico-disciplinari del MIUR: |
Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 06 - Scienze mediche > MED/04 - Patologia generale |
[error in script]
[error in script]
| Depositato il: |
14 Apr 2015 06:01 |
| Ultima modifica: |
07 Mag 2016 01:00 |
| URI: |
http://www.fedoa.unina.it/id/eprint/10511 |
| DOI: |
10.6092/UNINA/FEDOA/10511 |
Abstract
Changes of histone methylation code, cytosine hydroxylation and chromatin loop formation are associated with transcription induction by nuclear hormones, but it is not known if these events are the consequence or the cause of transcription initiation.
We show periodic oscillations of histone H3 methylation, G oxidation, C methylation and hydroxyl-methylation at the enhancer and polyA addition sites after induction of transcription in prototypic estrogen-induced genes. The oscillations correlate with formation of chromatin loops that juxtapose the 5' and 3' ends of the induced genes. BER followed by NER enzymes are recruited to the 5' and 3' end sites to repair the DNA oxidative lesions and to stabilize the 5' and 3' borders of the transcriptional units included in the loops. At the 5' end site of the loops, repair of oxidized Gs and hydroxymethylated Cs proceeds in a strand specific fashion.
We suggest that coupling transcription with methylation and repair enzymes is the evolutionary strategy to reduce the mutational burder induced by DNA oxidation at the borders of chromatin loops.
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