Montesano, Alessia (2016) CENTRAL REGULATION OF FOOD INTAKE PEPTIDES DURING AGEING IN THE TELEOST FISH NOTHOBRANCHIUS FURZERI. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: CENTRAL REGULATION OF FOOD INTAKE PEPTIDES DURING AGEING IN THE TELEOST FISH NOTHOBRANCHIUS FURZERI
Creators:
CreatorsEmail
Montesano, Alessiamontesanoalessia@gmail.com
Date: March 2016
Number of Pages: 131
Institution: Università degli Studi di Napoli Federico II
Department: Medicina Veterinaria e Produzioni Animali
Scuola di dottorato: Scienze veterinarie per la produzione e la sanità
Dottorato: Organismi modello nella ricerca biomedica e veterinaria
Ciclo di dottorato: 28
Coordinatore del Corso di dottorato:
nomeemail
de Girolamo, Paolodegirola@unina.it
Tutor:
nomeemail
de Girolamo, PaoloUNSPECIFIED
Date: March 2016
Number of Pages: 131
Uncontrolled Keywords: neuropeptides; food intake; ageing; Nothobranchius furzeri
Settori scientifico-disciplinari del MIUR: Area 07 - Scienze agrarie e veterinarie > VET/01 - Anatomia degli animali domestici
Area 07 - Scienze agrarie e veterinarie > VET/02 - Fisiologia veterinaria
Date Deposited: 12 Apr 2016 12:18
Last Modified: 20 May 2017 01:00
URI: http://www.fedoa.unina.it/id/eprint/10704

Abstract

In course of ageing, significant modifications occur to energy homeostasis in elderly subjects, representing often risk factors for morbidity and mortality. The mechanisms deputed to energetic control have been selected by ancestral diets, resulting from nutrient disposal during evolution. To better understand the regulation behind changes of appetite drive, we focus our study on the age-related expression of peptides specifically involved in the food intake process in the Central Nervous System (CNS). In fact, the appetite is modulated by a network of molecules balancing metabolic needs and caloric income. Specifically, we analyse the mRNA and protein expression levels of neuropeptide Y (NPY) and orexin (HCRT/ORX), both appetite stimulators, and nesfatin-1 (NUCB2), a hunger inhibitor. To evaluate the changes during ageing, we use the teleost fish Nothobranchius furzeri, that is an established model for ageing research, besides being the vertebrate with shortest lifespan described in captivity. We study starvation-induced neuropeptide regulation in the MZM-0410 and GRZ strains, at different ageing time-points (MZM: 5-27; GRZ: 5-12 weeks old), after food deprivation for 96 hours. We apply a multidisciplinary approach: the presence of the peptides in N. furzeri is confirmed by Western blot, gene expression is quantified by qPCR and the cells expressing mRNAs and neuropeptides are identified on brain sections. Further, the neuronal activation pattern after starvation is described by immunohistochemistry for phospho-S6 ribosomal protein. We show that during ageing NPY and NUCB2 are upregulated, whereas HCRT is downregulated; some differences exist between the two strains. Starvation determinates upregulation of NPY and HCRT, but just in young age. Fasting doesn’t cause variation of NUCB2 expression. Whereas, neurons expressing all three molecules are localised in neuroendocrine areas, where neuronal activation occurs. In conclusion, we present the first characterisation of NPY, HCRT and NUCB2 in N. furzeri, underlining their role as central modulator of food intake, as described in other vertebrates, but also highlighting some peculiarity of the two strains. Moreover, at present, this is the first analysis of food intake neuropeptides regulation upon ageing in a fish model.

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