Esposito, Francesco (2016) STUDY ON EXPOSURE TO ENDOCRINE DISRUPTORS AND MYCOTOXINS IN SUSCEPTIBLE POPULATION, THROUGH DIFFERENT COMPOUND-SPECIFIC CLEAN-UP METHODS AND APPLICATION OF GAS AND LIQUID CHROMATOGRAPHY COUPLED WITH FLUORESCENCE AND MASS SPECTROMETRY DETECTION. [Tesi di dottorato]

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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: STUDY ON EXPOSURE TO ENDOCRINE DISRUPTORS AND MYCOTOXINS IN SUSCEPTIBLE POPULATION, THROUGH DIFFERENT COMPOUND-SPECIFIC CLEAN-UP METHODS AND APPLICATION OF GAS AND LIQUID CHROMATOGRAPHY COUPLED WITH FLUORESCENCE AND MASS SPECTROMETRY DETECTION
Autori:
AutoreEmail
Esposito, Francescofrancesco.esposito4@unina.it
Data: 30 Marzo 2016
Numero di pagine: 62
Istituzione: Università degli Studi di Napoli Federico II
Dipartimento: Agraria
Scuola di dottorato: Scienze agrarie e agro-alimentari
Dottorato: Scienze e tecnologie delle produzioni agro-alimentari
Ciclo di dottorato: 28
Coordinatore del Corso di dottorato:
nomeemail
Barbieri, Giancarlobarbieri@unina.it
Tutor:
nomeemail
Cirillo, Teresa[non definito]
Data: 30 Marzo 2016
Numero di pagine: 62
Parole chiave: Endocrine disruptors; Fumonisins; Exposure assessment
Settori scientifico-disciplinari del MIUR: Area 06 - Scienze mediche > MED/42 - Igiene generale e applicat
Depositato il: 08 Apr 2016 09:25
Ultima modifica: 04 Nov 2016 08:45
URI: http://www.fedoa.unina.it/id/eprint/10816

Abstract

Chemical risk related to food safety is linked to the occurrence of potentially toxic chemicals in the food. Due to the different nature of the chemicals that may be present in food, a proper assessment of toxicological risk is crucial. This implies the need to establish an acceptable daily intake (ADI) and a tolerable daily intake (TDI) for residues of contaminants. The legal limits for toxic chemicals in food are based on careful assessments of intake levels of these compounds. However, certain populations could show higher exposure levels than the rest of the population, due both to the lower body weight (babies and children) and to a greater consumption of contaminated products. In recent years, there has been an increasing interest in the exposure to endocrine disruptors, substances that can compete with steroid hormones, thus interfering with normal endocrine functions. Among these compounds, phthalates and bisphenols are present in epoxy resins used in the internal coating of aluminum containers, as well as additive in plastics intended to come into contact with food. Foodstuffs, therefore, can be contaminated by these compounds through a migration from the container to the product. As regards natural toxicants, mycotoxins play a fundamental role in terms of chemical risk. In recent years, particular interest was shown in fumonisins, secondary metabolites of some fungal species belonging to the genus Fusarium, that can attack a variety of plants, including corn. The B1, B2 and B3 forms of these toxins have been classified by IARC as probably carcinogenic. Foods intend for people affected by celiac disease contain mainly corn, so the consumption of these products could pose a health concern in terms of daily exposure. Therefore objectives of this PhD work was the estimation of exposure to endocrine disruptors (di-ethylhexyl phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA)) of newborns, through the infants formulas consumption, and children from 3 to 10 years, through the consumption of soft drinks and detection of fumonisins in gluten-free foods, in order to estimate the exposure of children affected by celiac disease to such compounds. The infant formula samples showed a median level of total contamination equal to 1.136 µg / g dry weight (d.w.) for DEHP, 0.244 µg / g d.w. for DBP and 0.008 µg / g d.w. for BPA. The estimation of exposure to these contaminants showed that the effect on TDI issued by EFSA amounted up to 93% for DEHP, 175% for DBP and 25% for BPA. About children's exposure to BPA through the consumption of soft drinks, the exposure of the high consumers, was found to be equal to 44% of the EFSA TDI. The median total contamination levels were 0.79 µg / L. During this thesis an alternate clean-up method was evaluated for detection of bisphenols through cartridges based on Molecularly Imprinted Polymers (MIP), which led to satisfactory results in terms of recovery rates. Finally, exposure to fumonisins through consumption of gluten-free food could, in some cases, exceed by 30% the TDI issued by EFSA. In conclusion, canned sugary drinks can be a not negligible source of BPA. About infant formulas, the results showed a widespread contamination by DEHP, DBP and BPA. Such contamination seems to be due to environment contamination, rather than be related to a migration from the container. Due to matrix interferences that may occur during the analysis, the purification through MIP cartridges, even though more expensive, showed a great specificity for the bisphenols, with high recovery percentages, facilitating the determination of such compounds. As regards fumonisins, finally, further studies need to be carried out in order to evaluate also the exposure contribution of masked fumonisins.

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