Belviso, Immacolata (2017) In vitro evaluation of therapeutic potential of exosomes delivered by human cardiac primitive cells in cardiac regeneration. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: In vitro evaluation of therapeutic potential of exosomes delivered by human cardiac primitive cells in cardiac regeneration
Date: 3 April 2017
Number of Pages: 71
Institution: Università degli Studi di Napoli Federico II
Department: Sanità Pubblica
Scuola di dottorato: Medicina preventiva, pubblica e sociale
Dottorato: Sanità pubblica e medicina preventiva
Ciclo di dottorato: 29
Coordinatore del Corso di dottorato:
Castaldo, ClotildeUNSPECIFIED
Date: 3 April 2017
Number of Pages: 71
Uncontrolled Keywords: Exosomes; cardiac primitive cells; cardiac regeneration
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/16 - Anatomia umana
Date Deposited: 20 Apr 2017 10:25
Last Modified: 13 Mar 2018 11:38
DOI: 10.6093/UNINA/FEDOA/11493


Although, when injected, human Cardiac Primitive Cells (CPC) are not retained by host myocardium, they still improve cardiac function. Emerging evidence supports the hypothesis that exosomes may be responsible for beneficial effects induced by stem cells delivered in the infarcted myocardium. Exosomes are nano-sized vesicles naturally secreted by almost all cells and ubiquitously found in cell culture supernatants and biological fluids. Transporting and transferring peptides, lipids, and nucleic acids, exosomes have the potential to modulate signaling pathways, cell growth, migration, and proliferation of recipient cells. Accordingly, CPC may deliver chemoattractive, pro-survival and differentiating signals to resident cells through exosomes. To test our hypothesis, we isolated exosomes released in culture by CPC isolated from adult human myocardium (Exo-CPC) and analyzed the composition of their cargo and the effects elicited in vitro by their administration to resident population of CPC or fibroblasts. Specifically, we searched for the presence of specific factors known to regulate CPC migration, survival and differentiation. Additionally, we tested in vitro the potential of Exo-CPC of either regulating CPC proliferation and programmed cell death, and modulating interstitial fibrosis, extracellular-matrix (ECM) synthesis and deposition. Interestingly, on one hand, signals delivered by Exo-CPC affected proliferation and survival of CPC and, on the other hand, regulated ECM protein production. Therefore, we might speculate that Exo-CPC have potential effect on both resident CPC and fibroblasts when injected in cardiac wall.


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