Peirce, Sara (2017) Carbonic anhydrase biocatalysts for biomimetic CO2 capture. [Tesi di dottorato]


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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: Carbonic anhydrase biocatalysts for biomimetic CO2 capture
Data: 5 Aprile 2017
Numero di pagine: 129
Istituzione: Università degli Studi di Napoli Federico II
Dipartimento: Ingegneria Chimica, dei Materiali e della Produzione Industriale
Dottorato: Biotecnologie
Ciclo di dottorato: 29
Coordinatore del Corso di dottorato:
Salatino, Piero[non definito]
Marzocchella, Antonio[non definito]
Russo, Maria Elena[non definito]
Data: 5 Aprile 2017
Numero di pagine: 129
Parole chiave: CCS processes, enzyme immobilization techniques
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/10 - Biochimica
Area 09 - Ingegneria industriale e dell'informazione > ING-IND/25 - Impianti chimici
Depositato il: 18 Apr 2017 13:11
Ultima modifica: 08 Mar 2018 08:46
DOI: 10.6093/UNINA/FEDOA/11532


Novel post-combustion treatments include biomimetic CCS processes based on CO2 absorption into aqueous solutions assisted by enzyme catalysis. Carbonic anhydrase (CA) has been proposed as biocatalyst for biomimetic CCS: CA catalyses CO2 hydration at a turnover number ranging between 10^4 and 10^6 s^-1; it can be produced via fermentation and may be disposed of with minimal detrimental impact on the environment. The design/optimization of biomimetic CCS processes asks for the selection of proper CA forms because CA must be active and stable at the typical operating conditions of the CO2 capture unit (high temperature, pH and ionic strength, presence of pollutants) and should preserve a remarkable activity in the time. Design of the absorption unit (e.g. the reactor configuration) strongly depends on the use of the dissolved (homogeneous catalysis) or immobilized (heterogeneous catalysis) enzyme. The use of immobilized CA-based biocatalysts provides numerous advantages with respect to the use of the biocatalyst in dissolved form. Indeed, immobilized enzymes are characterized by significant increase of the enzyme stability at the process conditions. Morever, the use of solid biocatalysts allows the recovery and reuse of the enzyme from the liquid solvent. Whenever immobilized CA is used as CO2 absorption rate promoter, it should be active as close as possible to the gas-liquid interface to take advantage from the enzyme catalysis. This prerequisite may be fulfilled by a careful design of the morphology of the solid biocatalyst and selection of reactor configuration. The use of the slurry biocatalyst has been proposed as an optimal strategy. Indeed, the contribution of the catalytic fine particles to absorption rate enhancement is due to their ability to be available close to the gas–liquid interface. The present PhD thesis was focused on the development and characterization of CA-based biocatalysts to be used in innovative biomimetic absorption units. The activity was articulated as reported: (i) Development of protocols aimed to the production of solid CA-based biocatalysts, (ii) Kinetic characterization of a thermostable CA in free (homogeneous catalysis) and immobilized (heterogeneous catalysis) forms, under operating conditions close to those adopted in absorption units, such as temperature up to 313 K and carbonate-based solvents (≥ 10%wt). Moreover, a parallel activity was carried out and regarded the development of innovative enzymes co-immobilization techniques.

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