Mandracchia, Biagio (2017) Looking at the interface: Novel Microscopy Techniques for Quantitative Phase Imaging in Total Internal Reflection. [Tesi di dottorato]


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Item Type: Tesi di dottorato
Lingua: English
Title: Looking at the interface: Novel Microscopy Techniques for Quantitative Phase Imaging in Total Internal Reflection
Date: 7 April 2017
Number of Pages: 137
Institution: Università degli Studi di Napoli Federico II
Department: Ingegneria Chimica, dei Materiali e della Produzione Industriale
Dottorato: Ingegneria dei prodotti e dei processi industriali
Ciclo di dottorato: 29
Coordinatore del Corso di dottorato:
Mensitieri, GiuseppeUNSPECIFIED
Ferraro, PietroUNSPECIFIED
Date: 7 April 2017
Number of Pages: 137
Uncontrolled Keywords: Microscopy; Optics; Digital Holography;Total Internal Reflection; SPR;
Settori scientifico-disciplinari del MIUR: Area 02 - Scienze fisiche > FIS/01 - Fisica sperimentale
Area 02 - Scienze fisiche > FIS/07 - Fisica applicata (a beni culturali, ambientali, biologia e medicina)
Date Deposited: 25 Apr 2017 17:00
Last Modified: 08 Mar 2018 14:14
DOI: 10.6093/UNINA/FEDOA/11572


Looking at the interfaces is very important for many biological and chemical applications: from the imaging of thin polymeric films to the study of cell/substrate interactions. Total Internal Reflection (TIR) microscopy and Surface Plasmon Resonance imaging (SPRi) are usually the preferred techniques when high sensitivity of measurement is required. They use super critical illumination to create evanescent waves at the sample/substrate boundary. The very short range of penetration of these evanescent waves allows to retrieve information about few hundreds of nanometers from the contact surface. Evanescent wave microscopy has proven itself as a good tool for the characterization of thin films, cellular focal adhesions and biomolecular binding events. However, despite the high sensitivities achieved so far, each technique alone has a limited detection range with optimal sensitivity. The main goal of this work is to show how these techniques can benefit from the exploitation of the phase response of the evanescent waves, both in terms of sensitivity and reliability. In this thesis we show the versatility of Digital Holography Microscopy for the development of innovative and compact systems for quantitative phase imaging and, in particular, the implementation of through-the-objective configurations for Holographic TIR microscopy and SPRi. Advantages, issues, and applications are discussed throughout the work. At the same time, possible implementations and future perspectives are also presented with the aim to show the potential and raise interest for the development of new techniques for label-free imaging of interfaces.

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