Esposito, Francesca (2017) Transcoronary concentration gradients of circulating miRNAs in heart failure. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: Transcoronary concentration gradients of circulating miRNAs in heart failure
Creators:
CreatorsEmail
Esposito, Francescafrancescaesposito84@yahoo.it
Date: 28 November 2017
Number of Pages: 40
Institution: Università degli Studi di Napoli Federico II
Department: dep22
Dottorato: phd053
Ciclo di dottorato: 30
Coordinatore del Corso di dottorato:
nomeemail
Marone, Giannigianni.marone@unina.it
Tutor:
nomeemail
Rapacciuolo, AntonioUNSPECIFIED
Date: 28 November 2017
Number of Pages: 40
Uncontrolled Keywords: Heart failure, microRNA, biomarkers
Settori scientifico-disciplinari del MIUR: Area 06 - Scienze mediche > MED/11 - Malattie dell'apparato cardiovascolare
Date Deposited: 21 Dec 2017 09:10
Last Modified: 12 Apr 2019 09:11
URI: http://www.fedoa.unina.it/id/eprint/12020

Abstract

Circulating levels of microRNA (miRs) are emergent promising biomarkers for cardiovascular disease. Altered expression of miRs has been related to heart failure (HF) and cardiac remodeling. To identify the potential source for miRs released into the circulation, we measured the concentration gradients across the coronary circulation to assess their usefulness to diagnose HF of different etiologies. Circulating miRs were measured by TaqMan polymerase chain reaction in EDTA-plasma simultaneously obtained from the aorta (Ao) and the coronary venous sinus (CVS) in patients with non-ischemic HF (NICM-HF, n=23), or ischemic HF (ICM-HF, n=23), as well as from control subjects with no HF (n=11). We found differential modulation of circulating levels of the miRs 423, 34a, 21-3p and 126 across the etiology HF groups. Interestingly, we found a positive transcoronary gradient for the miR-423 (p<0.001) and the miR-34a (p<0.001), only in the ICM-HF group. On the contrary, a positive transcoronary gradient was found for the miR-21-3p (p<0.001) only in the NICM-HF group. Finally, despite the dramatic downregulation observed for the miR-126 in HF patients (p<0.001) compared to controls, no significant variations were observed in its transcoronary gradient. The present findings suggest that circulating levels of miRs are differentially expressed in patients with HF of different etiologies. The presence of a transcoronary concentration gradient suggests a selective release of miR by the failing heart into the coronary circulation. The presence of etiology-specific transcoronary concentration gradient in HF patients might provide important information to better understand their role in HF, and suggests they could be useful biomarkers to distinguish HF of different etiologies.

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