Coniglio, Antonio (2017) Epigenetic regulators HDA19 and ARP6 influence tomato development. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: Epigenetic regulators HDA19 and ARP6 influence tomato development
Creators:
CreatorsEmail
Coniglio, Antonioantonio.coniglio2@unina.it
Date: 8 December 2017
Number of Pages: 82
Institution: Università degli Studi di Napoli Federico II
Department: dep01
Dottorato: phd073
Ciclo di dottorato: 30
Coordinatore del Corso di dottorato:
nomeemail
D'Urso, Guidodurso@unina.it
Tutor:
nomeemail
Aversano, RiccardoUNSPECIFIED
Date: 8 December 2017
Number of Pages: 82
Uncontrolled Keywords: epigenetic; tomato development; ripening; acetylation; histone variant;
Settori scientifico-disciplinari del MIUR: Area 07 - Scienze agrarie e veterinarie > AGR/07 - Genetica agraria
Date Deposited: 27 Dec 2017 09:47
Last Modified: 08 Apr 2019 11:00
URI: http://www.fedoa.unina.it/id/eprint/12089

Abstract

Tomato as a fleshy fruit is one of the most important components of healthy diets, providing unique and critical contributions to food security. Fleshy fruit in comparison with dry, or dehiscent, fruit undergo a range of changes in chemistry and physiology, including synthesis and accumulation of characteristic pigments, evolution of aroma volatiles and modifications of texture with the final goal of spreading seeds through the attraction of animal vectors. Recently, increasing evidence has indicated that the regulatory network of tomato development include not only hormonal and genetic regulation but also epigenetic modulations. Substantial advances have been achieved in understanding DNA methylation, which plays a critical role as an important developmental regulatory component. However, in addition to DNA methylation, histone post-translational modifications (HPTMs) and histone variants can influence chromatin structure and gene expression. Among HPTMs, histone acetylation and deacetylation are biological processes considered crucial in plant growth and development through facilitation of chromatin relaxation and gene transcription regulation. Among histone variants, H2A.Z is the most evolutionary conserved and can impacts multiple processes, including transcription, DNA repair and response to environmental stresses. The ATP-dependent chromatin-remodeling complex SWR1 controls H2A.Z replacement in the nucleosome. However, HPTMs and nucleosome histone variants are not as well documented as DNA methylation in tomato plant development. Given that, our work has focused on the identification and the functional characterization, using amiRNA silenced mutants, of tomato Histone Deacetylase 19 (HDA19) and Actin Related Protein 6 (ARP6). The former is a histone deacetylase (HDACs) belonging to the RPD3 family and it has been shown to control flowering time, germination and seed set reduction in Arabidopsis. The latter is one of the subunits of the SWR1 complex and for that reason has been widely used to study the effects of H2A.Z depletion from chromatin. Arabidopsis plants defective in ARP6 exhibit global reduction in size, curly leaves, altered inflorescence and flower morphology, and early flowering. Our phenotypic analysis showed that HDA19 influences fruit size, ethylene production and carotenoids accumulation. In addition, HDA19 impacts on seeds set and is therefore necessary for embryo development. Conversely, ARP6 has a role on the vegetative development of tomato. It also influence germination and early seedlings development. Further, we showed that ARP6 contribute to plant tolerance to salt and heat stress in tomato. Taken together our data suggest a clear involvement of epiregulators HDA19 and ARP6 during reproductive and vegetative development of tomato, respectively.

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