Capasso, Renato and Cristinzio, Gennaro and Di Maro, Antimo and Ferranti, Pasquale and Parente, Augusto (2001) Syringicin, a new α-elicitin from an isolate of Phytophthora syringae, pathogenic to citrus fruit. [Pubblicazione in rivista scientifica]

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Item Type: Pubblicazione in rivista scientifica
Uncontrolled Keywords: Phytophthora syringae; citrus fruits; elicitins, amino acid sequence; syringicin, biological activity.
Date Deposited: 12 Jan 2009
Last Modified: 30 Apr 2014 19:25
URI: http://www.fedoa.unina.it/id/eprint/1219

Abstract

Syringicin (Syr), a new acidic α-elicitin, has been isolated from culture filtrates of Phytophthora syringae, which is the causal agent of citrus fruit rot. Its molecular weight (10194.6±0.2) determined by ES-MS is very similar to that calculated using the mean isotopic composition and considering the occurrence of disulfide bridges. The primary structure was obtained by the combined use of Edmann degradation with MALDI-MS and by comparison with already known a-elicitins. Syr induces a hypersensitive response and electrolyte leakage in tobacco. These are characteristic elicitor properties of the group and in agreement with the molecular mechanism recently proposed for this kind of protein. Finally, its possible applications in biological agriculture and biomedicine are briefly discussed.

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