fernandez, inigo (2018) BIOACTIVE CROSSLINKED PROTEIN-BASED FILMS REINFORCED BY NANOPARTICLES. [Tesi di dottorato]

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Item Type:	Tesi di dottorato
Lingua:	English
Title:	BIOACTIVE CROSSLINKED PROTEIN-BASED FILMS REINFORCED BY NANOPARTICLES
Creators:	Creators Email fernandez, inigo inigo.fernandez@unina.it
Date:	4 January 2018
Number of Pages:	91
Institution:	Università degli Studi di Napoli Federico II
Department:	dep19
Dottorato:	phd012
Ciclo di dottorato:	30
Coordinatore del Corso di dottorato:	nome email Sannia, Gioavanni sannia@unina.it
Tutor:	nome email Di Pierro, Prospero UNSPECIFIED
Date:	4 January 2018
Number of Pages:	91
Uncontrolled Keywords:	ACTIVE PACKAGING
Settori scientifico-disciplinari del MIUR:	Area 05 - Scienze biologiche > BIO/10 - Biochimica
Date Deposited:	15 Jan 2018 09:49
Last Modified:	14 Mar 2019 12:16
URI:	http://www.fedoa.unina.it/id/eprint/12268

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• BIOACTIVE CROSSLINKED PROTEIN-BASED FILMS REINFORCED BY NANOPARTICLES. (deposited 15 Jan 2018 09:49) [Currently Displayed]

Abstract

Edible films nanostructured with mesopouros silica or with its amino-functionalized derivative were prepared from seed bitter vetch proteins, before and after their crosslinking by microbial transglutaminase, and characterized for their physicochemical, morphological and bioactive properties. Film tensile strength and elongation at break significantly increased in the presence of both kinds of nanoparticles, even though the amino-functionalized ones resulted more effective, determining a two-fold increase of the mechanical properties, probably because of their ability to ionically interact with the negative moieties of the matrix proteins. Transglutaminase-catalyzed protein crosslinking counteracted these nanoparticle induced effects while, conversely, it further increased film barrier properties to gases and water vapour obtained by nanoparticles alone. AFM and SEM analyses indicated a more compact structure of the nanocomposite film matrix with more evident continuous zones compared to control films, as well as an effect of transglutaminase in including more homogenously both nanoparticles into the crosslinked protein network. Finally, all films exhibited antimicrobial and antifungal activities, probably due to phenolic compound(s) present in the bitter vetch protein concentrate, and the addition to the film forming solutions of the bioactive oligopeptide nisin significantly enhanced these properties.

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