Natale, Silvia (2018) Identification of the role played by Na+/Ca2+ exchanger 1 (NCX1) in hippocampal dependent anxiety, spatial learning and memory consolidation through genetic modified mice and newly synthesized compounds. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: English
Title: Identification of the role played by Na+/Ca2+ exchanger 1 (NCX1) in hippocampal dependent anxiety, spatial learning and memory consolidation through genetic modified mice and newly synthesized compounds.
Creators:
CreatorsEmail
Natale, Silviasilvia.natale@unina.it
Date: 4 December 2018
Number of Pages: 122
Institution: Università degli Studi di Napoli Federico II
Department: Neuroscienze e Scienze Riproduttive ed Odontostomatologiche
Dottorato: Neuroscienze
Ciclo di dottorato: 31
Coordinatore del Corso di dottorato:
nomeemail
Taglialatela, Mauriziomtaglial@unina.it
Tutor:
nomeemail
Molinaro, PasqualeUNSPECIFIED
Date: 4 December 2018
Number of Pages: 122
Uncontrolled Keywords: memory; anxiety, pharmacology
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 05 - Scienze biologiche > BIO/14 - Farmacologia
Area 05 - Scienze biologiche > BIO/15 - Biologia farmaceutica
Date Deposited: 19 Dec 2018 11:18
Last Modified: 30 Jun 2020 08:47
URI: http://www.fedoa.unina.it/id/eprint/12468

Abstract

The Na+/Ca2+ exchanger 1 (NCX1), which is highly expressed in the hippocampus, cortex, and amygdala, regulates Akt1 phosphorylation. Since Akt1 is part of the PTEN/Akt1/CREB signaling pathway, which participates in synaptic plasticity and long-term memory, we investigated whether NCX1 expression/activity might also be involved in some hippocampal-dependent learning and memory processes. To test this hypothesis, we used two genetically-modified mice to selectively overexpress (NCX1.4over) or downregulate (NCX1ko) NCX1 in hippocampal, cortical, and amygdala neurons and a newly synthesized selective NCX1 stimulating compound, named CN-PYB2. Both NCX1.4over and CN-PYB2-treated mice showed increased phosphorylated CaMKII levels in the hippocampus and improved long-term spatial learning and memory performance. By contrast, NCX1ko mice displayed a decrease in both. In addition, both NCX1.4over and CN-PYB2-treated mice also displayed an increase in context-dependent anxiety. Altogether, these results demonstrate that neuronal NCX1 participates in hippocampal-amygdala memory consolidation and promote anxiety behavior.

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