Lanzilli, Mariamichela (2018) Bacterial Spore: analysis of surface proteins and biotechnological applications. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Bacterial Spore: analysis of surface proteins and biotechnological applications |
| Autori: | Autore Email Lanzilli, Mariamichela mariamichelalanzilli@gmail.com |
| Data: | 8 Dicembre 2018 |
| Numero di pagine: | 162 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Biologia |
| Dottorato: | Biologia |
| Ciclo di dottorato: | 31 |
| Coordinatore del Corso di dottorato: | nome email Cozzolino, Salvatore salvatore.cozzolino@unina.it |
| Tutor: | nome email Isticato, Rachele [non definito] |
| Data: | 8 Dicembre 2018 |
| Numero di pagine: | 162 |
| Parole chiave: | Bacillus subtilis; B. megaterium; spores; drug delivery system; antioxidants |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/19 - Microbiologia generale |
| Depositato il: | 03 Gen 2019 14:21 |
| Ultima modifica: | 27 Giu 2020 05:09 |
| URI: | http://www.fedoa.unina.it/id/eprint/12511 |
Abstract
In my PhD project, I focused on two bacteria of the same genus, Bacillus subtilis and Bacillus megaterium. These organisms are Gram-positive, aerobic and spore formers. This work includes studies on the sporulation temperature consequences on the ultrastructure and the protein composition of the spore surface layers and on the functional properties of spores produced at growth temperature 25 and 42°C, in comparison to an optimal growth temperature (37°C). In addition, I focused on the possibility to use the bacterial spore as a platform to deliver Bio-Drugs to gastro-intestinal as they are able to stabilize and protect the adsorbed molecules by degradation and to ensure its activity. Moreover, the antioxidant properties of B.subtilis and B.megaterium spores have been also investigated analyzing the mechanism of action and the effects in vitro and in vivo.The results have showed that the spores are able to exert a preventive and therapeutic effect on the cells through the nuclear translocation of the transcriptional factor NRF-2, involved in the activation of responsive genes stress.
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