SCHETTINO, PIETRO (2018) L’utilizzo di un eptapeptide marcato con fluoresceina per l'identificazione della displasia colica, in pazienti affetti da rettocolite ulcerosa: studio pilota. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Lingua: Italiano
Title: L’utilizzo di un eptapeptide marcato con fluoresceina per l'identificazione della displasia colica, in pazienti affetti da rettocolite ulcerosa: studio pilota
Creators:
CreatorsEmail
SCHETTINO, PIETROpetrusschettino@gmail.com
Date: 2018
Number of Pages: 18
Institution: Università degli Studi di Napoli Federico II
Department: Medicina Clinica e Chirurgia
Dottorato: Terapie avanzate biomediche e chirirgiche
Ciclo di dottorato: 31
Coordinatore del Corso di dottorato:
nomeemail
DI MINNO, GIOVANNIdiminno@unina.it
Tutor:
nomeemail
DE PALMA, GIOVANNIUNSPECIFIED
Date: 2018
Number of Pages: 18
Uncontrolled Keywords: displasia, rettocolite ulcerosa, eptapeptide, endomicroscopia confocale laser
Settori scientifico-disciplinari del MIUR: Area 06 - Scienze mediche > MED/18 - Chirurgia generale
Date Deposited: 08 Jan 2019 08:32
Last Modified: 30 Jun 2020 09:13
URI: http://www.fedoa.unina.it/id/eprint/12623

Abstract

Aim Targeted molecular probes have been used to detect sporadic colonic dysplasia during confocal laser endomicroscopy (CLE) with promising results. This is a feasibility pilot study aiming to assess the potential role of CLE combined with a fluorescent-labeled peptide to stain and detect dysplasia associated with Ulcerative Colitis. Method A phage-derived heptapeptide with predicted high binding affinity for dysplastic tissue, was synthesized and labeled with fluorescein. Eleven lesions with suspected dysplasia at endoscopy were excised from nine patients with long-standing ulcerative colitis. Specimens were sprayed with the peptide and examined by CLE. The CLE images were then compared to the corresponding histological sections. Results At definitive histology, 4 lesions were diagnosed as inflammatory polyps, 6 as dysplastic lesions and one as invasive cancer. In inflammatory polyps, the fluorescence signal came from peri-cryptal spaces and crypt lumen due to passive accumulation of the peptide in these areas. Dysplasia was associated with active binding of the peptide to dysplastic colonocytes. Conclusion Ex vivo staining of ulcerative colitis-associated dysplasia using a fluorescent labeled molecular probe and CLE is feasible. In vivo studies on larger populations are required to evaluate the safety and the effective contribution of molecular probes in cancer surveillance of ulcerative colitis.

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