Ferraro, Giarita (2018) Interaction between proteins and bimetallic compounds of medicinal interest. [Tesi di dottorato]

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Tipologia del documento:	Tesi di dottorato
Lingua:	English
Titolo:	Interaction between proteins and bimetallic compounds of medicinal interest
Autori:	Autore Email Ferraro, Giarita giarita.ferraro@gmail.com
Data:	19 Dicembre 2018
Numero di pagine:	183
Istituzione:	Università degli Studi di Napoli Federico II
Dipartimento:	Scienze Chimiche
Dottorato:	Scienze chimiche
Ciclo di dottorato:	31
Coordinatore del Corso di dottorato:	nome email Paduano, Luigi luigi.paduano@unina.it
Tutor:	nome email Merlino, Antonello [non definito]
Data:	19 Dicembre 2018
Numero di pagine:	183
Parole chiave:	ferritin; drug-delivery; bimetallic compounds; cytotoxicity; selectivity
Settori scientifico-disciplinari del MIUR:	Area 03 - Scienze chimiche > CHIM/02 - Chimica fisica
Depositato il:	19 Gen 2019 16:24
Ultima modifica:	16 Giu 2020 09:00
URI:	http://www.fedoa.unina.it/id/eprint/12710

Abstract

Protein nanocages have attracted intense attention as drug delivery systems due to merits that include high biocompatibility, high solubility and ease of surface modification. Ferritin (Ft) nanocage has been used to encapsulate a variety of drugs and biologically active substances. Cytotoxic bimetallic compounds have been encapsulated within the Ft nanocage. Inductively plasma mass spectrometry, circular dichroism, UV-Vis absorption spectroscopy and X-ray crystallography confirm the encapsulation of these potential drugs. The structures of the adducts that these compounds form with Ft reveal that, upon encapsulation, in some cases the analysed bimetallic compounds can covalently bind the protein residues side chains , while in other cases do not interact directly with the protein. The biological activity of the drug-loaded nanocarriers has been tested: they have a cytotoxic effect on different human cancer cell lines, whereas they are significantly less cytotoxic for non-tumorigenic cells and exhibit much higher cytotoxicity than free AFt, which is basically non-toxic. The kind of cell death induced and the oxidative stress pathway activation have been deeply investigated.

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