Ferraro, Giarita (2018) Interaction between proteins and bimetallic compounds of medicinal interest. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Resource language: English
Title: Interaction between proteins and bimetallic compounds of medicinal interest
Creators:
CreatorsEmail
Ferraro, Giaritagiarita.ferraro@gmail.com
Date: 19 December 2018
Number of Pages: 183
Institution: Università degli Studi di Napoli Federico II
Department: Scienze Chimiche
Dottorato: Scienze chimiche
Ciclo di dottorato: 31
Coordinatore del Corso di dottorato:
nomeemail
Paduano, Luigiluigi.paduano@unina.it
Tutor:
nomeemail
Merlino, AntonelloUNSPECIFIED
Date: 19 December 2018
Number of Pages: 183
Keywords: ferritin; drug-delivery; bimetallic compounds; cytotoxicity; selectivity
Settori scientifico-disciplinari del MIUR: Area 03 - Scienze chimiche > CHIM/02 - Chimica fisica
Date Deposited: 19 Jan 2019 16:24
Last Modified: 16 Jun 2020 09:00
URI: http://www.fedoa.unina.it/id/eprint/12710

Collection description

Protein nanocages have attracted intense attention as drug delivery systems due to merits that include high biocompatibility, high solubility and ease of surface modification. Ferritin (Ft) nanocage has been used to encapsulate a variety of drugs and biologically active substances. Cytotoxic bimetallic compounds have been encapsulated within the Ft nanocage. Inductively plasma mass spectrometry, circular dichroism, UV-Vis absorption spectroscopy and X-ray crystallography confirm the encapsulation of these potential drugs. The structures of the adducts that these compounds form with Ft reveal that, upon encapsulation, in some cases the analysed bimetallic compounds can covalently bind the protein residues side chains , while in other cases do not interact directly with the protein. The biological activity of the drug-loaded nanocarriers has been tested: they have a cytotoxic effect on different human cancer cell lines, whereas they are significantly less cytotoxic for non-tumorigenic cells and exhibit much higher cytotoxicity than free AFt, which is basically non-toxic. The kind of cell death induced and the oxidative stress pathway activation have been deeply investigated.

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