Ferraro, Giarita (2018) Interaction between proteins and bimetallic compounds of medicinal interest. [Tesi di dottorato]
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| Item Type: | Tesi di dottorato |
|---|---|
| Resource language: | English |
| Title: | Interaction between proteins and bimetallic compounds of medicinal interest |
| Creators: | Creators Email Ferraro, Giarita giarita.ferraro@gmail.com |
| Date: | 19 December 2018 |
| Number of Pages: | 183 |
| Institution: | Università degli Studi di Napoli Federico II |
| Department: | Scienze Chimiche |
| Dottorato: | Scienze chimiche |
| Ciclo di dottorato: | 31 |
| Coordinatore del Corso di dottorato: | nome email Paduano, Luigi luigi.paduano@unina.it |
| Tutor: | nome email Merlino, Antonello UNSPECIFIED |
| Date: | 19 December 2018 |
| Number of Pages: | 183 |
| Keywords: | ferritin; drug-delivery; bimetallic compounds; cytotoxicity; selectivity |
| Settori scientifico-disciplinari del MIUR: | Area 03 - Scienze chimiche > CHIM/02 - Chimica fisica |
| Date Deposited: | 19 Jan 2019 16:24 |
| Last Modified: | 16 Jun 2020 09:00 |
| URI: | http://www.fedoa.unina.it/id/eprint/12710 |
Collection description
Protein nanocages have attracted intense attention as drug delivery systems due to merits that include high biocompatibility, high solubility and ease of surface modification. Ferritin (Ft) nanocage has been used to encapsulate a variety of drugs and biologically active substances. Cytotoxic bimetallic compounds have been encapsulated within the Ft nanocage. Inductively plasma mass spectrometry, circular dichroism, UV-Vis absorption spectroscopy and X-ray crystallography confirm the encapsulation of these potential drugs. The structures of the adducts that these compounds form with Ft reveal that, upon encapsulation, in some cases the analysed bimetallic compounds can covalently bind the protein residues side chains , while in other cases do not interact directly with the protein. The biological activity of the drug-loaded nanocarriers has been tested: they have a cytotoxic effect on different human cancer cell lines, whereas they are significantly less cytotoxic for non-tumorigenic cells and exhibit much higher cytotoxicity than free AFt, which is basically non-toxic. The kind of cell death induced and the oxidative stress pathway activation have been deeply investigated.
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