Cupo, Adelaide (2021) Heterotrophic cultivation of microalgae: metabolism and biotechnological applications. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Heterotrophic cultivation of microalgae: metabolism and biotechnological applications |
| Autori: | Autore Email Cupo, Adelaide Adelaide.Cupo@gmail.com |
| Data: | 26 Luglio 2021 |
| Numero di pagine: | 213 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Biologia |
| Dottorato: | Biologia |
| Ciclo di dottorato: | 33 |
| Coordinatore del Corso di dottorato: | nome email Cozzolino, Salvatore cozzolin@unina.it |
| Tutor: | nome email Esposito, Sergio [non definito] |
| Data: | 26 Luglio 2021 |
| Numero di pagine: | 213 |
| Parole chiave: | heterotrophy, diatoms, lipids |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/04 - Fisiologia vegetale |
| Depositato il: | 26 Lug 2021 12:23 |
| Ultima modifica: | 07 Giu 2023 11:25 |
| URI: | http://www.fedoa.unina.it/id/eprint/13555 |
Abstract
This PhD thesis aims to explore the biotechnological potential of the marine diatom Cyclotella cryptica grown under heterotrophic growth conditions. Diatoms are microorganisms generally cultivated in autotrophy, representing an important green source of valuable bio-products, like vitamins, pigments, omega-3 fatty acids and biofuels. However, there are significant limitations associated with autotrophic cultivation of microalgae on large scale, such as light supply and seasonal fluctuations, that affects overall costs. On these grounds, we decided to explore the heterotrophic growth condition that appears to be interesting for the development of commercial products of interest. In this way, all the light dependence constraints would be completely eliminated, by using the fermenter technology already consolidated in the pharmaceutical field at industrial scale. The process could be well controlled in terms of pH, temperature, addition and consumption of nutrients and maintenance of axenicity. The identification of candidate species and strains that have a high potential to grow in heterotrophy, as well as the improvement of culture conditions, represent a major challenge for biotechnology research. We chose Cyclotella cryptica as a model, due to its metabolic plasticity, robustness and the potential of obtainable products as reported in the Aquatic Species Program (ASP) funded by the US Department of Energy. The thesis is developed on several levels, conceptually grouped into three parts: the first one described the assessment of heterotrophic culture conditions and the behavior of C. cryptica grown in dark, the second part explores the potential products obtainable from the heterotrophic growth of C. cryptica and the last one give insights into the lipid pathway, through -omics analysis.
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