Di Crescenzo, Rosa Maria (2021) PROGNOSTIC ROLE OF CAF-1 COMPLEX IN UVEAL MELANOMA. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | PROGNOSTIC ROLE OF CAF-1 COMPLEX IN UVEAL MELANOMA |
| Autori: | Autore Email Di Crescenzo, Rosa Maria rosamariadicrescenzo@gmail.com |
| Data: | 13 Luglio 2021 |
| Numero di pagine: | 34 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Scienze Biomediche Avanzate |
| Dottorato: | Scienze biomorfologiche e chirurgiche |
| Ciclo di dottorato: | 33 |
| Coordinatore del Corso di dottorato: | nome email Cuocolo, Alberto cuocolo@unina.it |
| Tutor: | nome email Staibano, Stefania [non definito] |
| Data: | 13 Luglio 2021 |
| Numero di pagine: | 34 |
| Parole chiave: | Uveal Melanoma, CAF-1, Eye |
| Settori scientifico-disciplinari del MIUR: | Area 06 - Scienze mediche > MED/08 - Anatomia patologica |
| Depositato il: | 20 Lug 2021 08:40 |
| Ultima modifica: | 07 Giu 2023 10:44 |
| URI: | http://www.fedoa.unina.it/id/eprint/13636 |
Abstract
Uveal melanoma (UM) is the most common intraocular neoplasia in adults. Despite the recent advances in local therapy approaches, more than 50% of patients with UM still develop distant metastases, with a poor survival rate from the diagnosis of metastatic disease. Therefore, there is an urgent need to identify new reliable prognostic biomarkers and new therapeutic protocols for UM patients. Chromatin Assembly Factor-1 (CAF-1), a trimeric protein complex required for chromatin assembly after replication, which assembles histone octamers onto newly-replicated DNA, is an extremely promising new prognostic factor for solid malignancies of various histogenesis. We tested the immunohistochemical expression of CAF-1 p60 and p150 on paraffin-embedded tissue sections from 9 tissue microarrays (TMAs) made by cores, obtained from 133 UMs FFPE blocks. UMs included in this study were collected from 1990 to 2018, from the archives of the UOC of Surgical Pathology of the Federico II University of Naples and the Department G.F. Ingrassia, Section of Anatomic Pathology, University of Catania. We compared the immunohistochemical expression of CAF-1 subunits with BAP 1 expression, currently considered a relevant prognostic marker in UM. CAF-1/p60 and CAF-1/ p150 proteins were found overexpressed in our series of UM cases, with a significant correlation with an unfavorable outcome. Moreover, Immunohistochemical evaluation of BAP1 resulted less effective in prognostic stratification of patients. CAF-1/p60 and CAF-1/p150 resulted as reliable immunohistochemical markers, particularly in combination, to identify UM patients with a high risk of recurrence or metastasis. This finding opens up the chance of adding CAF-1 immunohistochemistry to the routine tissue prognostic algorithm for UM and provides new therapeutic insights.
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