Borzacchiello, Domenica (2021) Metabolic control of tumor growth by mitochondrial A-KINASE anchor protein 1 (AKAP1). [Tesi di dottorato]

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Item Type: Tesi di dottorato
Resource language: English
Title: Metabolic control of tumor growth by mitochondrial A-KINASE anchor protein 1 (AKAP1)
Creators:
CreatorsEmail
Borzacchiello, Domenicadomenica.borzacchiello@unina.it
Date: 11 July 2021
Number of Pages: 48
Institution: Università degli Studi di Napoli Federico II
Department: Medicina Molecolare e Biotecnologie Mediche
Dottorato: Medicina molecolare e biotecnologie mediche
Ciclo di dottorato: 33
Coordinatore del Corso di dottorato:
nomeemail
Santoro, Massimomassimo.santoro@unina.it
Tutor:
nomeemail
Feliciello, AntonioUNSPECIFIED
Avvedimento, Vittorio EnricoUNSPECIFIED
Date: 11 July 2021
Number of Pages: 48
Keywords: AKAP1, PKA, metabolism, DNA damage, mitochondria, cancer
Settori scientifico-disciplinari del MIUR: Area 06 - Scienze mediche > MED/04 - Patologia generale
Date Deposited: 19 Jul 2021 10:55
Last Modified: 07 Jun 2023 10:40
URI: http://www.fedoa.unina.it/id/eprint/13704

Collection description

Mitochondria are powerhouses present in all eukaryotic cells that play a fundamental role in energy production, survival and metabolism. In cancer cells, mitochondria provide the building blocks for the biogenesis of cellular organelles, cytoskeleton and membranes, and supply all the metabolic needs for cancer growth and spreading in vivo. AKAP1 is a scaffold protein that integrates and focus cAMP and src signaling on mitochondria, regulating protein synthesis, organelle biogenesis, oxidative metabolism and cell survival. During my thesis, I analyzed the mechanisms controlling the expression of AKAP1 in cancer cells and the role of this anchor protein in the control of metabolic pathways and cancer growth. I found that transcription and accumulation of AKAP1 are induced by the Myc proto-oncogene and by steroid hormones. I detected high levels of AKAP1 in a wide variety of high-grade human cancer tissues and cells, including prostate cancer, breast cancer and glioblastoma (GBM). I demonstrated that AKAP1 is required for mTOR pathway, oxidative metabolism and cancer growth, both in vitro and in vivo. Interestingly, I discovered a link between DNA damage pathways and AKAP1. In particular, I found that, in course of DNA damage, AKAP1 is phosphorylated by ATM/ATR kinase at its PKA binding domain. Phosphorylation of AKAP1 by ATM inhibits PKA targeting to mitochondria and downregulates oxidative metabolism. These data disclose a previously unrecognized role of AKAP1 in mTOR pathway and cancer growth AKAP1 and identify AKAP1 as a novel biologically relevant target of the DNA damage pathways.

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