Addeo, Martina (2021) Insight into Nephrocan function in mouse endoderm patterning. [Tesi di dottorato]

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Item Type: Tesi di dottorato
Resource language: English
Title: Insight into Nephrocan function in mouse endoderm patterning
Creators:
CreatorsEmail
Addeo, Martinamartina.addeo@gmail.com
Date: 8 July 2021
Number of Pages: 54
Institution: Università degli Studi di Napoli Federico II
Department: Biologia
Dottorato: Biologia applicata
Ciclo di dottorato: 33
Coordinatore del Corso di dottorato:
nomeemail
Cozzolino, Salvatorecozzolin@unina.it
Tutor:
nomeemail
Falco, GeppinoUNSPECIFIED
Date: 8 July 2021
Number of Pages: 54
Keywords: Endoderm development; embryonic stem cells; pancreatic differentiation.
Settori scientifico-disciplinari del MIUR: Area 05 - Scienze biologiche > BIO/13 - Biologia applicata
Date Deposited: 23 Jul 2021 10:48
Last Modified: 07 Jun 2023 10:48
URI: http://www.fedoa.unina.it/id/eprint/13720

Collection description

Endoderm-derived organs as the liver and pancreas are potential targets for regenerative therapies, and so there is great interest in understanding the pathways that regulate the induction and specification of this germ layer. Currently, the knowledge of molecular mechanisms that guide the in vivo endoderm specification is restricted by the lack of early endoderm-specific markers. Nephrocan (Nepn) is a gene whose expression characterizes the early stages of murine endoderm specification (E7.5-11.5) and encodes a secreted N-glycosylated protein. In the present study, we report the identification of a new transcript variant which is generated through alternative splicing. The new variant was found to have differential and tissue specific expression in the adult mouse. In order to better understand Nepn role during endoderm specification, we generated Nepn knock-out (KO) mice. Nepn-/- mice were born at Mendelian ratios and displayed no evident phenotype compared to WT mice. In addition, we produced a nullizygous mouse embryonic stem cell (mESC) line lacking Nepn by applying (CRISPR)/CRISPR-associated systems 9 (Cas9) and employed a differentiation protocol toward endoderm lineage. Our in vitro results revealed that Nepn loss affects the endoderm differentiation impairing the expression of posterior foregut-associated markers.

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