Muto, Tommaso (2022) T2-HIGH AND T2-LOW SEVERE ASTHMA: ROLE OF BASOPHIL ACTIVATION TEST (BAT). [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | T2-HIGH AND T2-LOW SEVERE ASTHMA: ROLE OF BASOPHIL ACTIVATION TEST (BAT) |
| Autori: | Autore Email Muto, Tommaso tommasomuto@gmail.com |
| Data: | 7 Marzo 2022 |
| Numero di pagine: | 63 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Medicina Clinica e Chirurgia |
| Dottorato: | Medicina clinica e sperimentale |
| Ciclo di dottorato: | 34 |
| Coordinatore del Corso di dottorato: | nome email Beguinot, francesco beguino@unina.it |
| Tutor: | nome email Marone, Gianni [non definito] |
| Data: | 7 Marzo 2022 |
| Numero di pagine: | 63 |
| Parole chiave: | ASTHMA BAT |
| Settori scientifico-disciplinari del MIUR: | Area 06 - Scienze mediche > MED/10 - Malattie dell'apparato respiratorio |
| Depositato il: | 17 Mar 2022 09:38 |
| Ultima modifica: | 28 Feb 2024 14:12 |
| URI: | http://www.fedoa.unina.it/id/eprint/14567 |
Abstract
Allergic disorders are one of the most common chronic diseases worldwide. Skin prick test and the measurement of immunoglobulin (Ig)E antibody are routinely used for the diagnosis of allergic disorders. However, skin prick test and serum IgE detect only sensitization but do not prove the clinical manifestations of allergic disease. Allergic disorders (i.e., asthma, allergic rhinitis, urticaria, atopic dermatitis, anaphylaxis) include a variety of immediate IgE-mediated hypersensitivity reactions. They are characterized by the abnormal reaction to allergens, which, in sensitized subjects, induce IgE-mediated degranulation of human basophils and mast cells with the release of several proinflammatory mediators. The basophil activation test (BAT) mimics in vitro the allergic reaction and has manifold advantages as diagnostic test: safety profile, sensitivity/specificity, and the potential to predict the severity of an allergic reaction. There is some evidence that BAT allows the discrimination between sensitized asymptomatic subjects and truly allergic individuals and has the potential to replace expensive and risky in vivo allergen bronchoprovocation tests The aim of our study was to evaluate, in two groups of severe asthmatic patients (T2-high and T2-low), the ability of anti-IgE and of the Staphylococcus aureus enterotoxins to induce the activation of peripheral blood basophils, using the BAT. This technique employes flow cytometry to demonstrate in vitro the degranulation of basophils from asthmatic patients and control subjects following the activation with different stimuli. Peripheral blood basophils are identified as CRTH2+ and CD3- cells. The activation of basophils was evaluated through the expression of CD203c. Our results indicate that anti-IgE induced CD203c basophil expression is increased in T2-high and T2-low asthmatics compared to healthy controls. Staphylococcal enterotoxins can induce the activation of basophils only in a percentage of patients with severe asthma.
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