Ricciardi, Simona (2023) The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle |
| Autori: | Autore Email Ricciardi, Simona simona.ricciardi@unina.it |
| Data: | 5 Marzo 2023 |
| Numero di pagine: | 111 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Medicina Molecolare e Biotecnologie Mediche |
| Dottorato: | Medicina molecolare e biotecnologie mediche |
| Ciclo di dottorato: | 35 |
| Coordinatore del Corso di dottorato: | nome email Santoro, Massimo masantor@unina.it |
| Tutor: | nome email De Matteis, Maria Antonietta [non definito] |
| Data: | 5 Marzo 2023 |
| Numero di pagine: | 111 |
| Parole chiave: | COVID19; Replication; Replication Organelle; Double membrane vesicles; Endoplasmic Reticulum; viral protein. |
| Settori scientifico-disciplinari del MIUR: | Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 05 - Scienze biologiche > BIO/13 - Biologia applicata |
| Depositato il: | 21 Mar 2023 10:29 |
| Ultima modifica: | 10 Apr 2025 14:13 |
| URI: | http://www.fedoa.unina.it/id/eprint/15205 |
Abstract
Two-thirds of human coronaviruses genome translates for two overlapping open reading frames (ORF1a and ORF1b), which generate two polyproteins (pp1a and pp1ab) to generate all the components needed for the replication. The polyproteins are then further processed by two viral proteases to generate 16 nonstructural proteins (nsp1-16), which are involved in different stages of the replication cycle. Of these, the nonstructural membrane proteins NSP3, NSP4, and NSP6, have been shown to play critical roles in cell membrane rearrangement, promoting the formation of replication organelles (ROs). In this compartment, viral RNA synthesis occurs. Many aspects of this process are still unclear, mainly how SARS-CoV-2 can use the cellular machinery to perform its functions.Therefore, we analyzed the process of RO biogenesis by expressing NSP3, NSP4, and NSP6 proteins alone or in combination. In particular, while the role in the formation of DMVs has been reported for NSP3 and NSP4, we focused on analyzing the unidentified role of NSP6.
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