Tantipongpiradet, Ariyawan Investigation on Bacopa monnieri (L.) Wetts. and Camellia sinensis (L.) Kuntze, alone and in combination, as candidates for potential neurotherapeutic agents. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Investigation on Bacopa monnieri (L.) Wetts. and Camellia sinensis (L.) Kuntze, alone and in combination, as candidates for potential neurotherapeutic agents |
| Autori: | Autore Email Tantipongpiradet, Ariyawan ariyawan.tantipongpiradet@unina.it |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Farmacia |
| Dottorato: | Nutraceuticals, Functional Foods and Human Healt |
| Ciclo di dottorato: | 35 |
| Coordinatore del Corso di dottorato: | nome email RITIENI, ALBERTO alberto.ritieni@unina.it |
| Tutor: | nome email DAGLIA, MARIA [non definito] |
| Parole chiave: | Bacopa monnieri, Camellia sinensis, Neurotherapeutic agents, Combination therapy, Neuroinflammation, Neuroprotective, In viro |
| Depositato il: | 27 Mar 2023 11:29 |
| Ultima modifica: | 10 Apr 2025 14:26 |
| URI: | http://www.fedoa.unina.it/id/eprint/15247 |
Abstract
Bacopa monnieri (BM) and Camellia sinensis (CS) are natural plants used as nutraceutical products with neuroprotective properties. BM is a memory and learning enhancer (Brimson et al., 2021), while CS is a supplement with high antioxidant activity. Several previous studies have investigated the neurotherapeutic effect on a single extract, but there is currently no evidence regarding the combination of BM and CS. This study was a preliminary investigation to explore the effects of BM and CS, both alone and in combination, on microglial activation, and to explore their antioxidant potential in neurons. Using lipopolysaccharide (LPS)-stimulated BV2 microglia as an in vitro model of neuroinflammation, we report that both extracts can significantly attenuate the expression of pro-inflammatory cytokines TNF-α and Interleukin-6, and the chemokine MCP-1, in a concentration-dependent manner as well as the combination of BM and CS. Further, nitrite concentration (Griess assay) and iNOS expression (Western immunoblot analysis) reveal that both test agents, alone and in combination, can significantly attenuate microglial nitric oxide (NO) production in response to LPS. Interestingly, the combination of BM and CS reduced H2O2-induced cytotoxicity in N2a cells and ameliorated neuroinflammatory reactions in LPS-stimulated microglia BV2 cells via inhibitions of NF-кB and MAPK with an additive effect.
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