Forgione, Maria Concetta (2024) Binding studies between Neisseria gonorrhoeae lipooligosaccharides (LOS) and monoclonal antibodies. [Tesi di dottorato]
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Binding studies between Neisseria gonorrhoeae lipooligosaccharides (LOS) and monoclonal antibodies |
| Autori: | Autore Email Forgione, Maria Concetta mariaconcetta.forgione@unina.it |
| Data: | 11 Marzo 2024 |
| Numero di pagine: | 162 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Scienze Chimiche |
| Dottorato: | Scienze chimiche |
| Ciclo di dottorato: | 36 |
| Coordinatore del Corso di dottorato: | nome email Lombardi, Angelina alombard@unina.it |
| Tutor: | nome email Silipo, Alba [non definito] |
| Data: | 11 Marzo 2024 |
| Numero di pagine: | 162 |
| Parole chiave: | Neisseria gonorrhoeae, lipooligosaccharide (LOS), monoclonal antibodies (MAb), epitope mapping, NMR spectroscopy, Mass Spectrometry, STD (Saturation Transfer Difference)-NMR, Cytoadhesins, Mycoplasma genitalium (Mge), Mycoplasma pneumoniae (Mpn), Sialoglycans, Molecular Dynamic Simulations (MD simulations); |
| Settori scientifico-disciplinari del MIUR: | Area 03 - Scienze chimiche > CHIM/06 - Chimica organica |
| Depositato il: | 21 Mar 2024 10:46 |
| Ultima modifica: | 16 Mar 2026 09:55 |
| URI: | http://www.fedoa.unina.it/id/eprint/15436 |
Abstract
Neisseria gonorrhoeae (Ng) is the etiological agent of gonorrhoea, a sexually transmitted disease (STI), which causes genital tract infection in male and female. Ng is a Gram-negative bacterium belonging to the diplococci family. It is well known that gonorrhoea represents a serious health problem, due to its worldwide spread, but also because of the uncontrolled increase in antibiotic resistance. The development of alternative and more targeted therapeutic treatments, such as a vaccine, is therefore urgently needed. One of the main immune targets is lipooligosaccharide (LOS), which is widely expressed on the outer membrane of Ng. LOS plays a key role in many biological processes, and it is in fact involved in recognition mechanisms, pathogenesis and the immune system's evasion mechanism. Therefore, the understanding at the molecular level of the interaction processes between LOSs and its receptors is crucial for developing new and more effective therapeutic strategies. The present thesis work reports the NMR characterization of the oligosaccharides (OSs) extracted from gonococcal strains FA1090 and 15253 and the interaction study between OSs and the monoclonal antibody 2C7. The second topic is focused on Mycoplasma genitalium (Mge) and Mycoplasma pneumoniae (Mpn), two human pathogen bacteria. Mge is associated with urogenital tract infections and causes urethritis, cervicitis and pelvic inflammatory disease. While Mpn is responsible for the upper and lower respiratory tract infections. Bacterial cytoadhesins, exposed on pathogen cell surface, as P140 and P110 in Mge, and their homologs P1 and P40/P90 in Mpn are essential for the interaction with the host, through the recognition of glycans decorating the cell surface and facilitate the bacterial adhesion and invasion. Thus, an accurate comprehension of the host-bacterium adhesion mechanisms can facilitate the exploration of novel anti-adherence therapies. Within this frame, the analysis of host sialoglycans recognition by P110 (Mge) and P40/P90 (Mpn) employing NMR Spectroscopy and computational studies has been reported.
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