Castanò, Ilenia
(2008)
“MicroRNA Expression Profiling in human
Subcutaneous Adipose Tissue”.
[Tesi di dottorato]
(Unpublished)
Item Type: |
Tesi di dottorato
|
Lingua: |
English |
Title: |
“MicroRNA Expression Profiling in human
Subcutaneous Adipose Tissue” |
Creators: |
Creators | Email |
---|
Castanò, Ilenia | yleniacastano@libero.it |
|
Date: |
9 November 2008 |
Number of Pages: |
60 |
Institution: |
Università degli Studi di Napoli Federico II |
Department: |
Biochimica e biotecnologie mediche |
Dottorato: |
Genetica e medicina molecolare |
Ciclo di dottorato: |
21 |
Coordinatore del Corso di dottorato: |
nome | email |
---|
Bruni, Carmelo Bruno | brucar@unina.it |
|
Tutor: |
nome | email |
---|
Pastore, Lucio | pastore@dbbm.unina.it |
|
Date: |
9 November 2008 |
Number of Pages: |
60 |
Uncontrolled Keywords: |
Obesity, MicroRNA, gene expression |
Settori scientifico-disciplinari del MIUR: |
Area 05 - Scienze biologiche > BIO/12 - Biochimica clinica e biologia molecolare clinica |
[error in script]
[error in script]
Date Deposited: |
05 Nov 2009 13:14 |
Last Modified: |
02 Dec 2014 11:42 |
URI: |
http://www.fedoa.unina.it/id/eprint/3031 |
DOI: |
10.6092/UNINA/FEDOA/3031 |

Abstract
MicroRNA are believed to be generally involved in more quantitative regulation of
mammalian trait: therefore, mammalian conditions that have a strong genetic
component and involve quantitative differences between the physiological and
pathological conditions are strong candidates to be influenced by miRNA
disregulations. The pool of this study is thus to investigate the possible role for miRNA
imbalance in adipose tissue in the development of obesity.
To date, microRNAs have been identified and evaluated in human preadipocytes (miR-
143) and mouse hepatocytes (miR-122). There is no study dealing with microRNA in
subcutaneous adipose tissue (SAT) of obese patients.
For this reason, we have performed an high-throughput screening by microarray
analysis on 1,458 microRNAs in SAT from 20 morbidly obese patients (10 men and 10
female) versus pools of RNA from non obese subjects (3 men and 5 female).
We were able to identify miRNAs differentially expressed comparing SAT from obese
patients with respect to SAT from non-obese patients.
The miR-519d was significantly higher in obese subjects than in the non obese pools.
Using bioinformatic and functional analyses, we have demonstrated that the peroxisome
proliferator activated receptor A (PPARA) gene resulted be a target of miR-519d.
PPARA regulates the genes involved in fatty acid, glucose metabolism and
inflammation (Cho et al., 2008). Therefore, it is conceivable that loss of PPARA could
cause metabolic imbalance, thereby resulting in adipocyte hypertrophy in SAT from
morbidly obese subjects.
This project set the goal to be a part of the effort in understanding the biology and the
role of this miR-519d during biogenesis of obesity.
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