Banfi, Stefano and Barbieri, Paola and Bernasconi, Silvana and Bertini, Laura and Bertoni, Giovanni and Bianchi, Luca and Cafaro, Valeria and Caporale, Carlo and Caruso, Carla and Caruso, Enrico and Di Donato, Alberto and Di Gennaro, Patrizia and Donadio, Giuliana and Fanali, Gabriella and Fasano, Mauro and Giachetta, Rossella and Galli, Enrica and Izzo, Viviana and Lanfranchi, Marco and Mapelli, Erica and Notomista, Eugenio and Orlandi, Roberta and Orlandi, Viviana and Pennacchio, Francesca and Proietti, Silvia and Renzi, Francesco and Rescalli, Emanuela and Scognamiglio, Roberta and Sello, Guido and Troncone, Luca and Vaghi, Ivan and Varcamonti, Mario and Zonca, Cristina (2010) Metabolism and molecular systems for the biotransformation of aromatic molecules. Annual Project Meeting: Naples, 19 January 2010. In: Metabolism and molecular systems for the biotransformation of aromatic molecules, 19 January 2010, Naples.

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Item Type:	Conference or Workshop Item (Paper)
Title:	Metabolism and molecular systems for the biotransformation of aromatic molecules. Annual Project Meeting: Naples, 19 January 2010
Creators:	Creators Email Banfi, Stefano UNSPECIFIED Barbieri, Paola paola.barbieri@uninsubria.it Bernasconi, Silvana UNSPECIFIED Bertini, Laura UNSPECIFIED Bertoni, Giovanni giovanni.bertoni@unimi.it Bianchi, Luca UNSPECIFIED Cafaro, Valeria vcafaro@unina.it Caporale, Carlo caporale@unitus.it Caruso, Carla caruso@unitus.it Caruso, Enrico UNSPECIFIED Di Donato, Alberto didonato@unina.it Di Gennaro, Patrizia patrizia.digennaro@unimib.it Donadio, Giuliana UNSPECIFIED Fanali, Gabriella UNSPECIFIED Fasano, Mauro UNSPECIFIED Giachetta, Rossella UNSPECIFIED Galli, Enrica enrica.galli@unimi.it Izzo, Viviana vizzo@unina.it Lanfranchi, Marco UNSPECIFIED Mapelli, Erica UNSPECIFIED Notomista, Eugenio notomist@unina.it Orlandi, Roberta UNSPECIFIED Orlandi, Viviana UNSPECIFIED Pennacchio, Francesca UNSPECIFIED Proietti, Silvia UNSPECIFIED Renzi, Francesco UNSPECIFIED Rescalli, Emanuela UNSPECIFIED Scognamiglio, Roberta robscogn@unina.it Sello, Guido guido.sello@unimi.it Troncone, Luca UNSPECIFIED Vaghi, Ivan UNSPECIFIED Varcamonti, Mario UNSPECIFIED Zonca, Cristina varcamon@unina.it
Date:	19 January 2010
Date Type:	Completion
Number of Pages:	72
Event Type:	Workshop
Event Title:	Metabolism and molecular systems for the biotransformation of aromatic molecules
Event Location:	Naples
Event Dates:	19 January 2010
Date:	19 January 2010
Number of Pages:	72
Uncontrolled Keywords:	phe operon, tou operon, xyl-like genes, aldolase/dehydrogenase bifunctional enzyme, transcriptional regulation, toluene/o-xylene mono-oxygenase, site direct mutagenesis, biotransformations, biocatalysts, whole cell, multienzymatic, one-pot, organic compounds, synthesis, multienzymatic, antioxidants, Bioremediation, Novosphingobium, Sphingomonadales, fuel oil, polycyclic aromatic hydrocarbon, Pseudomonas putida, MvaT-like proteins, microarray analysis, Naphthalene, phenanthrene, oxygenase
Settori scientifico-disciplinari del MIUR:	Area 03 - Scienze chimiche > CHIM/06 - Chimica organica Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 05 - Scienze biologiche > BIO/10 - Biochimica Area 03 - Scienze chimiche > CHIM/11 - Chimica e biotecnologia delle fermentazioni Area 05 - Scienze biologiche > BIO/19 - Microbiologia generale
Date Deposited:	14 Apr 2010 12:44
Last Modified:	11 Sep 2014 11:01
URI:	http://www.fedoa.unina.it/id/eprint/3695

Abstract

The collection of papers is the result of the first year's results of the Project: METABOLISM AND MOLECULAR SYSTEMS FOR THE BIOTRANSFORMATION OF AROMATIC MOLECULES. Aim of the research project is to gain knowledge in microorganisms endowed with the ability of transforming aromatic compounds, and in the genes and enzymes involved in this process. Particular attention is paid to the information acquired for its use in the development of biosynthetic systems for bioremediation and bioconversions. The pathways responsible for the aerobic catabolism of aromatic molecules are generally made up of an upper pathway, where hydroxylation reactions leading to the formation of hydroxylated intermediates occur, and a lower pathway where the aromatic ring of these dihydroxylated molecules are cleaved, and eventually converted, after several enzymatic steps, into intermediates of the Krebs cycle. It goes without saying that the great potential of these systems is related to the acquisition of information about the enzymatic systems involved and the regulation of their corresponding genes. The utilization of these micro-organisms can be expanded and improved by: (i) characterizing new microorganisms and their enzymatic systems to sort out a wide panel of enzymatic systems endowed with different metabolic abilities and different specificity, (ii) elucidating the transcriptional regulation and the molecular determinants responsible for the enzymatic activities in systems that are already known and in those that will be available during the development of the project. Moreover, in order to evaluate the influence of the information gained in developing processes it will be necessary to (iii) validate the development of a small number of applications. More specifically these goals have been achieved through: 1) Isolation and characterization of new bacterial strains either by isolation from polluted sites or by spotting new ones through the analysis of annotated genomes for identifying both new microorganisms and new enzymatic activities with enhanced catalytic biodegradative properties; 2) identification and analysis of the molecular determinants of the specificity of enzymatic systems either already available or new ones which will be available during the development of the project; 3) investigation of the transcription regulative systems involved in the expression of the enzymatic systems (in particular key enzymes in the metabolism of aromatic compounds) in both psychrophilic and mesophilic bacteria; 4) utilization of the results obtained from the different approaches above to set-up new biocatalysts for the bioremediation of model wastewaters and for the biosynthesis of compounds of pharmaceutical and industrial interest.

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