Esposito, Carla Lucia (2009) RNA-based aptamers as high affinity ligands for tumor cell surface epitopes". [Tesi di dottorato] (Unpublished)

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Item Type: Tesi di dottorato
Language: English
Title: RNA-based aptamers as high affinity ligands for tumor cell surface epitopes"
Creators:
CreatorsEmail
Esposito, Carla Luciacarlaluciaesposito@libero.it
Date: 28 November 2009
Number of Pages: 148
Institution: Università degli Studi di Napoli Federico II
Department: Biologia e patologia cellullare e molecolare "L. Califano"
Doctoral School: Medicina molecolare
PHD name: Patologia e fisiopatologia molecolare
PHD cycle: 22
PHD Coordinator:
nameemail
Avvedimento, Vittorio EnricoUNSPECIFIED
Tutor:
nameemail
De Franciscis, Vittoriodefranci@unina.it
Date: 28 November 2009
Number of Pages: 148
Uncontrolled Keywords: Aptamers, SELEX, Cancer
MIUR S.S.D.: Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 06 - Scienze mediche > MED/04 - Patologia generale
Date Deposited: 28 May 2010 12:33
Last Modified: 30 Apr 2014 19:39
URI: http://www.fedoa.unina.it/id/eprint/3953

Abstract

Structured single-stranded nucleic acids, or aptamers, bind target molecules ranging from small chemical compounds to cells and tissues with high affinity and specificity. Thanks to their unique characteristics (low size, good affinity for the target, no immunogenicity, chemical structures that can be easily modified to improve their in vivo applications), aptamers are perfectly suitable to different areas of biotechnology. Currently, several aptamers are in the clinical pipeline for applications and their functional repertoire has expanded with aptamers as reagents for the targeted delivery. Here we demonstrate that aptamers are efficient tools to bind/inhibit important cell surface epitopes and can be used in clinical diagnosis and therapy. We have developed and validated an in vitro evolution-based approach, named differential whole cell SELEX, to generate a panel of high affinity RNA-based aptamers as ligands for a specific cancer cell phenotype (in two different tumor model systems, glioma and NSCLC). We demonstrate that such an approach can be effective for the generation of functional oligonucleotide ligands that are potentially suitable for diagnostic and therapeutic applications.

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