Esposito, Carla Lucia (2009) RNA-based aptamers as high affinity ligands for tumor cell surface epitopes". [Tesi di dottorato] (Inedito)

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Tipologia del documento:	Tesi di dottorato
Lingua:	English
Titolo:	RNA-based aptamers as high affinity ligands for tumor cell surface epitopes"
Autori:	Autore Email Esposito, Carla Lucia carlaluciaesposito@libero.it
Data:	28 Novembre 2009
Numero di pagine:	148
Istituzione:	Università degli Studi di Napoli Federico II
Dipartimento:	Biologia e patologia cellullare e molecolare "L. Califano"
Scuola di dottorato:	Medicina molecolare
Dottorato:	Patologia e fisiopatologia molecolare
Ciclo di dottorato:	22
Coordinatore del Corso di dottorato:	nome email Avvedimento, Vittorio Enrico [non definito]
Tutor:	nome email De Franciscis, Vittorio defranci@unina.it
Data:	28 Novembre 2009
Numero di pagine:	148
Parole chiave:	Aptamers, SELEX, Cancer
Settori scientifico-disciplinari del MIUR:	Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare Area 06 - Scienze mediche > MED/04 - Patologia generale
Depositato il:	28 Mag 2010 12:33
Ultima modifica:	30 Apr 2014 19:39
URI:	http://www.fedoa.unina.it/id/eprint/3953

Abstract

Structured single-stranded nucleic acids, or aptamers, bind target molecules ranging from small chemical compounds to cells and tissues with high affinity and specificity. Thanks to their unique characteristics (low size, good affinity for the target, no immunogenicity, chemical structures that can be easily modified to improve their in vivo applications), aptamers are perfectly suitable to different areas of biotechnology. Currently, several aptamers are in the clinical pipeline for applications and their functional repertoire has expanded with aptamers as reagents for the targeted delivery. Here we demonstrate that aptamers are efficient tools to bind/inhibit important cell surface epitopes and can be used in clinical diagnosis and therapy. We have developed and validated an in vitro evolution-based approach, named differential whole cell SELEX, to generate a panel of high affinity RNA-based aptamers as ligands for a specific cancer cell phenotype (in two different tumor model systems, glioma and NSCLC). We demonstrate that such an approach can be effective for the generation of functional oligonucleotide ligands that are potentially suitable for diagnostic and therapeutic applications.

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