Characterization and serotype distribution of Aggregatibacter actinomycetemcomitans detected in a population of periodontitis patients in Spain.

Blasi, Andrea (2009) Characterization and serotype distribution of Aggregatibacter actinomycetemcomitans detected in a population of periodontitis patients in Spain. [Tesi di dottorato] (Inedito)

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Abstract

Objective: To investigate the serotype distribution of Aggregatibacter actinomycetemcomitans (A. a.) and the polymorphism of the genes that codify the leukotoxin (ltx) and the operon of the cytolethal distending toxin (cdt) in periodontitis patients in Spain. Materials and methods: Forty A. a.-positive periodontitis patients were included in the study (mean age 45±7.8 years, 62.5 % female) and their clinical periodontal status was assessed. On average, 1 to 2 isolates from each patient were subcultured and characterized by specific PCR reactions. Results: Serotype distribution analysis concludes that the most frequent serotype was a and that 29 patients showed monoinfection. The 11 patients that showed coinfection were infected mostly with serotypes a and b. From these 40 isolates 15 corresponded to serotype a, 8 to serotype b and 6 to serotype c. Monoinfection with serotypes d, e or f was not found. Further characterization of these samples with leukotoxin specific PCR reaction showed that none of the 40 samples presented the 530-bp deletion called Δ530 in the leukotoxin’s promoter region, which characterizes the JP2 strain. As for the Cdt, the operon that codifies for the toxin was present in 85 % of the samples. Conclusions: The most common serotypes were a and b. Serotype a was the most frequent in monoinfections, while serotypes d, e and f were not detected. In the majority of samples, operon that codifies the cdt (85%) and the genes responsible for the codification of leukotoxin (100%) were found. None of the isolates belonged to the JP2 strain.

Tipologia di documento:Tesi di dottorato
Parole chiave:periodontitis, aggregatibacter actinomycetemcomitans, spain, serotype, leukotoxin, cdt, pcr
Settori scientifico-disciplinari MIUR:Area 06 Scienze mediche > MED/28 MALATTIE ODONTOSTOMATOLOGICHE
Coordinatori della Scuola di dottorato:
Coordinatore del Corso di dottoratoe-mail (se nota)
Rengo, Sandrosanrengo@unina.it
Tutor della Scuola di dottorato:
Tutor del Corso di dottoratoe-mail (se nota)
Matarasso, Sergiomatarass@unina.it
Stato del full text:Accessibile
Data:30 Novembre 2009
Numero di pagine:42
Istituzione:Università degli Studi di Napoli Federico II
Dipartimento o Struttura:Scienze Odontostomatologiche e Maxillo-Facciali
Tipo di tesi:Dottorato
Stato dell'Eprint:Inedito
Scuola di dottorato:Scuola di dottorato in medicina clinica e sperimentale
Denominazione del dottorato:Scienze Odontostomatologiche
Ciclo di dottorato:XXII
Numero di sistema:4239
Depositato il:01 Dicembre 2009 13:30
Ultima modifica:04 Agosto 2010 14:24

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