Blasi, Andrea (2009) Characterization and serotype distribution of Aggregatibacter actinomycetemcomitans detected in a population of periodontitis patients in Spain. [Tesi di dottorato] (Inedito)

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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: Characterization and serotype distribution of Aggregatibacter actinomycetemcomitans detected in a population of periodontitis patients in Spain.
Autori:
AutoreEmail
Blasi, Andrea[non definito]
Data: 30 Novembre 2009
Numero di pagine: 42
Istituzione: Università degli Studi di Napoli Federico II
Dipartimento: Scienze odontostomatologiche e maxillo-facciali
Scuola di dottorato: Medicina clinica e sperimentale
Dottorato: Scienze odontostomatologiche
Ciclo di dottorato: 22
Coordinatore del Corso di dottorato:
nomeemail
Rengo, Sandrosanrengo@unina.it
Tutor:
nomeemail
Matarasso, Sergiomatarass@unina.it
Data: 30 Novembre 2009
Numero di pagine: 42
Parole chiave: periodontitis, aggregatibacter actinomycetemcomitans, spain, serotype, leukotoxin, cdt, pcr
Settori scientifico-disciplinari del MIUR: Area 06 - Scienze mediche > MED/28 - Malattie odontostomatologiche
Depositato il: 01 Dic 2009 12:30
Ultima modifica: 30 Apr 2014 19:40
URI: http://www.fedoa.unina.it/id/eprint/4239
DOI: 10.6092/UNINA/FEDOA/4239

Abstract

Objective: To investigate the serotype distribution of Aggregatibacter actinomycetemcomitans (A. a.) and the polymorphism of the genes that codify the leukotoxin (ltx) and the operon of the cytolethal distending toxin (cdt) in periodontitis patients in Spain. Materials and methods: Forty A. a.-positive periodontitis patients were included in the study (mean age 45±7.8 years, 62.5 % female) and their clinical periodontal status was assessed. On average, 1 to 2 isolates from each patient were subcultured and characterized by specific PCR reactions. Results: Serotype distribution analysis concludes that the most frequent serotype was a and that 29 patients showed monoinfection. The 11 patients that showed coinfection were infected mostly with serotypes a and b. From these 40 isolates 15 corresponded to serotype a, 8 to serotype b and 6 to serotype c. Monoinfection with serotypes d, e or f was not found. Further characterization of these samples with leukotoxin specific PCR reaction showed that none of the 40 samples presented the 530-bp deletion called Δ530 in the leukotoxin’s promoter region, which characterizes the JP2 strain. As for the Cdt, the operon that codifies for the toxin was present in 85 % of the samples. Conclusions: The most common serotypes were a and b. Serotype a was the most frequent in monoinfections, while serotypes d, e and f were not detected. In the majority of samples, operon that codifies the cdt (85%) and the genes responsible for the codification of leukotoxin (100%) were found. None of the isolates belonged to the JP2 strain.

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