Ruggiero, Luca (2006) Molecular and functional characterization of the interaction between c-Myc and the MCM helicase. [Tesi di dottorato] (Unpublished)

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Item Type: Tesi di dottorato
Language: English
Title: Molecular and functional characterization of the interaction between c-Myc and the MCM helicase
Creators:
CreatorsEmail
Ruggiero, LucaUNSPECIFIED
Date: 2006
Date Type: Publication
Number of Pages: 99
Institution: Università degli Studi di Napoli Federico II
Department: Biologia e patologia cellullare e molecolare "L. Califano"
PHD name: Genetica e medicina molecolare
PHD cycle: 17
PHD Coordinator:
nameemail
Bruni, Carmelo BrunoUNSPECIFIED
Tutor:
nameemail
Lania, LuigiUNSPECIFIED
Date: 2006
Number of Pages: 99
Uncontrolled Keywords: Myc, Mcm, Replication
MIUR S.S.D.: Area 05 - Scienze biologiche > BIO/11 - Biologia molecolare
Area 06 - Scienze mediche > MED/03 - Genetica medica
Area 05 - Scienze biologiche > BIO/13 - Biologia applicata
Area 05 - Scienze biologiche > BIO/10 - Biochimica
Area 06 - Scienze mediche > MED/07 - Microbiologia e microbiologia clinica
Area 05 - Scienze biologiche > BIO/12 - Biochimica clinica e biologia molecolare clinica
Area 05 - Scienze biologiche > BIO/18 - Genetica
Date Deposited: 31 Jul 2008
Last Modified: 30 Apr 2014 19:23
URI: http://www.fedoa.unina.it/id/eprint/556

Abstract

The Myc genes (c-myc, n-myc, l-myc, s-myc) encode a family of protein that share structural an biological features and are conserved across all Metazoans. c-Myc, the most extensively studied of his family, has been found deregulated, at different level, in almost every kind of tumor. Originally described as a weak transcriptional activator, further studies disclosed uncommon features for an oncogene or a classic transcription factor. c-Myc exerts his influence on the cell growth, cell cycle progression, apoptosis, but the mechanisms that underlie these functions are still not well understood. In order to shed light on its role as master regulator of cell fate, we isolated the protein complex containing c-Myc. Among the molecular partners associated with c-Myc that were not described before, we have found Mcm7 and Mcm5. Mcm7 and Mcm5 are members of the MCM complex, a ring-shape structure composed by six proteins (Mcm2-7). They play a key role in DNA replication and are recently substituting the traditional tumor marker in clinical diagnosis. We have characterized the interaction between c-Myc and the entire MCM complex. Our studies seem to discard the possibility of a functional meaning of this interaction in c-Myc-mediated transcriptional activation, supporting the hypothesis of a direct effect of c-Myc in replication, already suggested by other authors.

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