Borriello, Marianna
(2010)
A Novel Fully Human Antitumor ImmunoRNase Targeting ErbB2-Positive Tumors.
[Tesi di dottorato]
(Inedito)
| Tipologia del documento: |
Tesi di dottorato
|
| Lingua: |
English |
| Titolo: |
A Novel Fully Human Antitumor ImmunoRNase Targeting ErbB2-Positive Tumors |
| Autori: |
| Autore | Email |
|---|
| Borriello, Marianna | marianna.borriello@gmail.com |
|
| Data: |
25 Novembre 2010 |
| Numero di pagine: |
39 |
| Istituzione: |
Università degli Studi di Napoli Federico II |
| Dipartimento: |
Biologia strutturale e funzionale |
| Scuola di dottorato: |
Scienze biologiche |
| Dottorato: |
Biochimica e biologia cellulare e molecolare |
| Ciclo di dottorato: |
23 |
| Coordinatore del Corso di dottorato: |
| nome | email |
|---|
| Arcari, Paolo | arcari@unina.it |
|
| Tutor: |
| nome | email |
|---|
| De Lorenzo, Claudia | cladelo@unina.it | | D'Alessio, Giuseppe | dalessio@unina.it |
|
| Data: |
25 Novembre 2010 |
| Numero di pagine: |
39 |
| Parole chiave: |
antitumor; ImmunoRNase; ErbB2; Erb-hcAb-RNase |
| Settori scientifico-disciplinari del MIUR: |
Area 05 - Scienze biologiche > BIO/10 - Biochimica |
[error in script]
[error in script]
| Depositato il: |
09 Dic 2010 16:47 |
| Ultima modifica: |
30 Apr 2014 19:43 |
| URI: |
http://www.fedoa.unina.it/id/eprint/7963 |
| DOI: |
10.6092/UNINA/FEDOA/7963 |
Abstract
A second generation anti-ErbB2 ImmunoRNase, called Erb-hcAb-RNase, was obtained by the fusion of Erb-hcAb, a human compact anti-ErbB2 antibody, with human pancreatic ribonuclease (HP-RNase or RNase 1).
We show herein that Erb-hcAb-RNase retains the enzymatic activity of human pancreatic RNase and specifically binds to ErbB2-positive cells with an affinity comparable to that of the parental Erb-hcAb. Moreover, this novel immunoRNase is endowed with an effective and selective antiproliferative action for ErbB2-positive tumor cells both in vitro and in vivo. Its antitumor activity is more potent than that of the parental Erb-hcAb as the novel immunoconjugate has acquired RNase-based cytotoxicity in addition to the inhibitory growth effects, antibody-dependent and complement-dependent cytotoxicity of the compact antibody.
Erb-hcAb-RNase could be a promising candidate for the immunotherapy of ErbB2-positive tumours as it combines the advantages of the first generation scFv-based immunoRNase with those of a fully functional antibody
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