Armellino, Carla (2010) Effects of probiotics, drugs and metallic ions on diarrhea and their mechanisms. [Tesi di dottorato] (Inedito)

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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: Effects of probiotics, drugs and metallic ions on diarrhea and their mechanisms
Autori:
AutoreEmail
Armellino, Carlacarlaarmellino@libero.it
Data: 30 Novembre 2010
Numero di pagine: 103
Istituzione: Università degli Studi di Napoli Federico II
Dipartimento: Pediatria
Scuola di dottorato: Medicina clinica e sperimentale
Dottorato: Riproduzione, sviluppo ed accrescimento dell'uomo
Ciclo di dottorato: 23
Coordinatore del Corso di dottorato:
nomeemail
Pignata, Claudiopignata@unina.it
Tutor:
nomeemail
Alfredo, Guarinoalfguari@unina.it
Data: 30 Novembre 2010
Numero di pagine: 103
Parole chiave: Diarrhea
Settori scientifico-disciplinari del MIUR: Area 06 - Scienze mediche > MED/17 - Malattie infettive
Area 06 - Scienze mediche > MED/38 - Pediatria generale e specialistica
Area 06 - Scienze mediche > MED/12 - Gastroenterologia
Depositato il: 03 Dic 2010 09:40
Ultima modifica: 30 Apr 2014 19:45
URI: http://www.fedoa.unina.it/id/eprint/8248

Abstract

Infection disease induces two mechanisms, ion secretion which results from secretion of chloride from the basolateral to apical side of enterocyte and osmotic diarrhea which is the consequence of massive flux of water from the basolateral to the luminal side upon the osmotic gradient originating by non absorbed intestinal nutrients. In both osmotic or secretory diarrhea, the intestine reacts with homeostatic mechanisms to increase ion absorption. These mechanisms can be effectively exploited to counteract fluid loss. A model of intestinal infections using rotavirus (RV) in epithelial cells was set up and used to define a model of secretory and osmotic diarrhea in intestinal cell line in vitro. Using this model, antidiarrheal drugs were tested, namely zinc , Thiorphan and probiotics. Zinc in vitro decreased the ion secretion induced by RV-induced enterotoxic activity. Thiorphan showed a potent and selective inhibition of active secretion, being particularly effective in the first phase of RV diarrhea. The probiotics, Lactobacillus GG and Saccharomyces boulardii, are able to reduce RV induced Teer decrease and RV cytotoxic effect with different mechanism by inducing cell growth and differentiation. The use of drugs selectively active at the intestinal level, therefore, provide an effective options to treat intestinal infections in childhood.

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