Armellino, Carla (2010) Effects of probiotics, drugs and metallic ions on diarrhea and their mechanisms. [Tesi di dottorato] (Unpublished)

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Item Type: Tesi di dottorato
Language: English
Title: Effects of probiotics, drugs and metallic ions on diarrhea and their mechanisms
Date: 30 November 2010
Number of Pages: 103
Institution: Università degli Studi di Napoli Federico II
Department: Pediatria
Doctoral School: Medicina clinica e sperimentale
PHD name: Riproduzione, sviluppo ed accrescimento dell'uomo
PHD cycle: 23
PHD Coordinator:
Date: 30 November 2010
Number of Pages: 103
Uncontrolled Keywords: Diarrhea
MIUR S.S.D.: Area 06 - Scienze mediche > MED/17 - Malattie infettive
Area 06 - Scienze mediche > MED/38 - Pediatria generale e specialistica
Area 06 - Scienze mediche > MED/12 - Gastroenterologia
Date Deposited: 03 Dec 2010 09:40
Last Modified: 30 Apr 2014 19:45


Infection disease induces two mechanisms, ion secretion which results from secretion of chloride from the basolateral to apical side of enterocyte and osmotic diarrhea which is the consequence of massive flux of water from the basolateral to the luminal side upon the osmotic gradient originating by non absorbed intestinal nutrients. In both osmotic or secretory diarrhea, the intestine reacts with homeostatic mechanisms to increase ion absorption. These mechanisms can be effectively exploited to counteract fluid loss. A model of intestinal infections using rotavirus (RV) in epithelial cells was set up and used to define a model of secretory and osmotic diarrhea in intestinal cell line in vitro. Using this model, antidiarrheal drugs were tested, namely zinc , Thiorphan and probiotics. Zinc in vitro decreased the ion secretion induced by RV-induced enterotoxic activity. Thiorphan showed a potent and selective inhibition of active secretion, being particularly effective in the first phase of RV diarrhea. The probiotics, Lactobacillus GG and Saccharomyces boulardii, are able to reduce RV induced Teer decrease and RV cytotoxic effect with different mechanism by inducing cell growth and differentiation. The use of drugs selectively active at the intestinal level, therefore, provide an effective options to treat intestinal infections in childhood.

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