The translational repressor Cup is required for germ plasm assembly and germ cell development in Drosophila.

Ottone, Cristina (2011) The translational repressor Cup is required for germ plasm assembly and germ cell development in Drosophila. [Tesi di dottorato] (Inedito)

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Abstract

RNA localization is a cellular mechanism used to localize proteins to sub-cellular domains and to control protein synthesis regionally. In oocytes, RNA localization has profound implications for development, generating asymmetric protein distributions that promote morphological and functional cell polarization and establish regional fates in the future embryo. One such fate is that of the germ cell lineage. In Drosophila, germ cell formation depends on maternal inherited factors localized in the posterior pole region of oocytes and early embryos, named germ plasm. Oskar (Osk), a key determinant of germ plasm assembly, is both necessary and sufficient for germ-line formation and posterior patterning The localization of oskar (osk) mRNA starts during oogenesis and it is mediated by trans-acting factors several of which have been shown to have roles in post-transcriptional regulation of RNA, such as splicing, translational control and degradation. However, most of the molecular mechanisms underlying osk mRNA localization are still unclear (Zhou Y. and King ML, 2004). During the course of my study, I have demonstrated that Cup, a translational regulator of specific mRNAs, localizes to both nuage, a germ-line perinuclear organelle assembled during early oogenesis, and germ plasm. Moreover, Cup protein interacts with Osk and Vasa (Vas) to assure anchoring, stabilization and/or maintenance of germ plasm particles to the posterior pole of oocytes and early embryos. According to these results, homozygous cup mutant embryos display a reduced number of germ cells, respect to the heterozygous cup mutants. The latest embryos exhibit in turn less germ cells than wild type. Finally, cup and osk interact genetically, since reducing cup copy number further decreases the total number of germ cells observed in heterozygous osk mutant embryos. In this thesis, I shed light on a novel role of Cup during germ plasm assembly and germ cell development in Drosophila.

Tipologia di documento:Tesi di dottorato
Altre informazioni:EUROPEAN SCHOOL OF MOLECULAR MEDICINE SEDE DI NAPOLI -SEMM - UNIVERSITA’ DEGLI STUDI DI NAPOLI “FEDERICO II” Ph.D. in Molecular Medicine – Ciclo IV/XXII Human Genetics CEINGE
Parole chiave:Germ cells, Cup, Drosophila
Settori scientifico-disciplinari MIUR:Area 05 Scienze biologiche > BIO/11 BIOLOGIA MOLECOLARE
Coordinatori della Scuola di dottorato:
Coordinatore del Corso di dottoratoe-mail (se nota)
Salvatore, Francesco
Tutor della Scuola di dottorato:
Tutor del Corso di dottoratoe-mail (se nota)
Cortese, Riccardo
Russo, Tommaso
Giangrande, Angela
Stato del full text:Accessibile
Data:30 Gennaio 2011
Numero di pagine:116
Istituzione:Università degli studi di Napoli Federico II
Dipartimento o Struttura:Biochimica e biotecnologie mediche
Tipo di tesi:Dottorato
Stato dell'Eprint:Inedito
Scuola di dottorato:Medicina molecolare
Denominazione del dottorato:Molecular Medicine
Ciclo di dottorato:22
Numero di sistema:8447
Depositato il:16 Febbraio 2011 14:28
Ultima modifica:29 Agosto 2012 13:43

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