Brancaccio, Diego (2011) Interaction of peptides and small molecules with biological targets. [Tesi di dottorato] (Inedito)

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Tipologia del documento: Tesi di dottorato
Lingua: English
Titolo: Interaction of peptides and small molecules with biological targets
Autori:
AutoreEmail
Brancaccio, Diegodiego.brancaccio@unina.it
Data: 30 Novembre 2011
Numero di pagine: 249
Istituzione: Università degli Studi di Napoli Federico II
Dipartimento: Chimica farmaceutica e tossicologica
Scuola di dottorato: Scienze farmaceutiche
Dottorato: Scienza del farmaco
Ciclo di dottorato: 24
Coordinatore del Corso di dottorato:
nomeemail
D'Auria, Maria Valeriamadauria@unina.it
Tutor:
nomeemail
Carotenuto, Alfonsoalfonso.carotenuto@unina.it
Data: 30 Novembre 2011
Numero di pagine: 249
Parole chiave: NMR, peptide, conformational analysis
Settori scientifico-disciplinari del MIUR: Area 03 - Scienze chimiche > CHIM/08 - Chimica farmaceutica
Depositato il: 06 Dic 2011 11:27
Ultima modifica: 30 Apr 2014 19:48
URI: http://www.fedoa.unina.it/id/eprint/8816
DOI: 10.6092/UNINA/FEDOA/8816

Abstract

During my PhD course, I focused my attention on the conformational analysis of peptides that interact with GPCR and ligand-receptor interactions from NMR spectroscopy with possible application to cytotoxic agents binding to DNA. In particular, I investigated the conformational behaviour of peptide analogues of Urotensin, Melanocortin and Somatostatin in water solution and membrane mimetic environment (SDS and DPC micelles). Another research field was the NMR-based screening as potent technique for the identification of small molecules that interact with macromolecule targets. Several methods based on the ligand observation have been proposed in the literature, among these WaterLOGSY (water-ligand observed via gradient spectroscopy), and STD-NMR (saturation transfer difference) experiments. I applied these NMR techniques for the evaluation of the DNA interactions of a new series of thiophen-naphthoquinones with interesting cytotoxic activity.

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