Brancaccio, Diego (2011) Interaction of peptides and small molecules with biological targets. [Tesi di dottorato] (Inedito)
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| Tipologia del documento: | Tesi di dottorato |
|---|---|
| Lingua: | English |
| Titolo: | Interaction of peptides and small molecules with biological targets |
| Autori: | Autore Email Brancaccio, Diego diego.brancaccio@unina.it |
| Data: | 30 Novembre 2011 |
| Numero di pagine: | 249 |
| Istituzione: | Università degli Studi di Napoli Federico II |
| Dipartimento: | Chimica farmaceutica e tossicologica |
| Scuola di dottorato: | Scienze farmaceutiche |
| Dottorato: | Scienza del farmaco |
| Ciclo di dottorato: | 24 |
| Coordinatore del Corso di dottorato: | nome email D'Auria, Maria Valeria madauria@unina.it |
| Tutor: | nome email Carotenuto, Alfonso alfonso.carotenuto@unina.it |
| Data: | 30 Novembre 2011 |
| Numero di pagine: | 249 |
| Parole chiave: | NMR, peptide, conformational analysis |
| Settori scientifico-disciplinari del MIUR: | Area 03 - Scienze chimiche > CHIM/08 - Chimica farmaceutica |
| Depositato il: | 06 Dic 2011 11:27 |
| Ultima modifica: | 30 Apr 2014 19:48 |
| URI: | http://www.fedoa.unina.it/id/eprint/8816 |
| DOI: | 10.6092/UNINA/FEDOA/8816 |
Abstract
During my PhD course, I focused my attention on the conformational analysis of peptides that interact with GPCR and ligand-receptor interactions from NMR spectroscopy with possible application to cytotoxic agents binding to DNA. In particular, I investigated the conformational behaviour of peptide analogues of Urotensin, Melanocortin and Somatostatin in water solution and membrane mimetic environment (SDS and DPC micelles). Another research field was the NMR-based screening as potent technique for the identification of small molecules that interact with macromolecule targets. Several methods based on the ligand observation have been proposed in the literature, among these WaterLOGSY (water-ligand observed via gradient spectroscopy), and STD-NMR (saturation transfer difference) experiments. I applied these NMR techniques for the evaluation of the DNA interactions of a new series of thiophen-naphthoquinones with interesting cytotoxic activity.
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